{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:27:01Z","timestamp":1773289621422,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,2,10]],"date-time":"2019-02-10T00:00:00Z","timestamp":1549756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["3\/2\/2\/39\/2010\/NCD\u2010III"],"award-info":[{"award-number":["3\/2\/2\/39\/2010\/NCD\u2010III"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Asia-Pac J Clncl Oncology"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>We investigated prognostic significance of methylation status of MASPIN gene and its protein expression in normal subjects, cholelithiasis and gallbladder cancer (GBC) patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>MASPIN protein expression and its promoter methylation in gallbladder tissue of cholelithiasis (<jats:italic>n<\/jats:italic>\u00a0=\u00a036), GBC (<jats:italic>n<\/jats:italic>\u00a0=\u00a046) and controls (<jats:italic>n<\/jats:italic>\u00a0=\u00a025) were investigated. Clinicopathological parameters and prognosis of patients with GBC were correlated with protein expression of MASPIN. Survival analysis of GBC patients was performed using Kaplan\u2013Meier method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Significant increased (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001) protein expression of MASPIN was observed in GBC as compared to cholelithiasis, whereas negligible expression was found in normal tissues. Methylation\u2010specific PCR revealed statistical significant (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.005) difference in methylation status of MASPIN promoter between gallstone and GBC patients. Significant association was observed between methylation profile with protein expression of MASPIN (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.004), stage (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.011) and cellular differentiation (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.012) for GBC. Also, significant (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.004) difference in survival was observed for malignant patients having demethylated MASPIN promoter. Further significant negative correlation (Pearson's coefficient [<jats:italic>r<\/jats:italic>]\u00a0=\u00a0\u22120.617; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001) was observed between MASPIN expression and survival of cancer patients after surgery. Overall survival was significantly shorter (<jats:italic>P<\/jats:italic>\u00a0\u2264\u00a00.0001; hazard ratio [HR] for death\u00a0=\u00a02.84; 95% confidence interval [CI],\u00a00.09865\u20130.3683) in patients of GBC with MASPIN expression \u2265169.56\u00a0pg\/mg (median survival; 10\u00a0months) with compared to those with expression &lt;169.56\u00a0pg\/mg (median survival; 16\u00a0months).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Overexpression of MASPIN protein may play an important role in malignant progression and is correlated with a poor prognosis. Also MASPIN DNA methylation can be used as a novel therapeutic target for treating GBC.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ajco.13129","type":"journal-article","created":{"date-parts":[[2019,2,11]],"date-time":"2019-02-11T04:23:57Z","timestamp":1549859037000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Significance of methylation status of MASPIN gene and its protein expression in prognosis of gallbladder cancer"],"prefix":"10.1111","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1977-7437","authenticated-orcid":false,"given":"Kavita","family":"Baghel","sequence":"first","affiliation":[{"name":"Department of Surgical Gastroenterology King George's Medical University  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hasan Raza","family":"Kazmi","sequence":"additional","affiliation":[{"name":"Department of Surgical Gastroenterology King George's Medical University  Lucknow India"},{"name":"Department of Biomedical Sciences PennVet University of Pennsylvania  Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abhijit","family":"Chandra","sequence":"additional","affiliation":[{"name":"Department of Surgical Gastroenterology King George's Medical University  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saloni","family":"Raj","sequence":"additional","affiliation":[{"name":"Department of Surgical Gastroenterology King George's Medical University  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajeshwar Nath","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery King George's Medical University  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/625695"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-014-0167-5"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.7314\/APJCP.2014.15.1.343"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)47379-6"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.21.11669"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.2.499"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203535"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1319"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3816\/CLC.2008.n.052"},{"key":"e_1_2_10_11_1","first-page":"812","article-title":"Expression of the tumor suppressor gene maspin in human pancreatic cancers","volume":"7","author":"Maass N","year":"2001","journal-title":"Clin Cancer Res"},{"key":"e_1_2_10_12_1","first-page":"2924","article-title":"The paradoxical expression of maspin in ovarian carcinoma","volume":"8","author":"Sood AK","year":"2002","journal-title":"Clin Cancer Res"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700214"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-010-0985-4"},{"key":"e_1_2_10_15_1","first-page":"532","article-title":"A reagent for the single\u2010step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples","volume":"15","author":"Chomczynski P","year":"1993","journal-title":"Biotechniques"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207211"},{"key":"e_1_2_10_17_1","first-page":"4771","article-title":"The surface of prostate carcinoma DU145 cells mediates the inhibition of urokinase\u2010type plasminogen activator by maspin","volume":"60","author":"McGowen R","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/72303"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205507"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.2.4.471"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20721"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0063581"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2005.12.025"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-010-0056-2"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol_00000110"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(03)01021-0"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-1589"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63549-3"},{"key":"e_1_2_10_29_1","first-page":"4158","article-title":"Frequent hypomethylation of multiple genes overexpressed in pancreatic ductal adenocarcinoma","volume":"15","author":"Sato N","year":"2003","journal-title":"Cancer Res"}],"container-title":["Asia-Pacific Journal of Clinical Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fajco.13129","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ajco.13129","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ajco.13129","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ajco.13129","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T07:41:40Z","timestamp":1694331700000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ajco.13129"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,10]]},"references-count":28,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["10.1111\/ajco.13129"],"URL":"https:\/\/doi.org\/10.1111\/ajco.13129","archive":["Portico"],"relation":{},"ISSN":["1743-7555","1743-7563"],"issn-type":[{"value":"1743-7555","type":"print"},{"value":"1743-7563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,10]]},"assertion":[{"value":"2018-04-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}