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Human prostate cancer xenograft models were exposed to ultrasound and transfection efficiency and histological examination were analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with the control group, ultrasound combined with microbubbles significantly improves gene transfection efficiency (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). In in vitro study, ultrasound combined with microbubbles resulted in cell lysis and the interruption of cell membrane continuity, and its average transfection efficiency was 9.9%; the green fluorescence intensity was 15.2% in the ultrasound combined with microbubbles group in vivo; both values were higher than that in the control group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Low\u2010frequency ultrasound combined with microbubbles could be used as a method to promote gene transfection in prostate cancer cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ajco.13521","type":"journal-article","created":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T05:43:11Z","timestamp":1614577391000},"page":"93-98","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Low\u2010frequency ultrasound combined with microbubbles improves gene transfection in 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