{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T23:36:02Z","timestamp":1787614562634,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,12,4]],"date-time":"2022-12-04T00:00:00Z","timestamp":1670112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["12171285"],"award-info":[{"award-number":["12171285"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["11971269"],"award-info":[{"award-number":["11971269"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Asia-Pac J Clncl Oncology"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To investigate the role of iodine\u2010125 (<jats:sup>125<\/jats:sup>I)\u00a0combined with epirubicin (EPI) in inhibiting hepatocellular carcinoma (HCC) growth and promoting apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Both in vivo and in vitro experiments were conducted. CCK\u20108 assay was performed to determine the cells viability after EPI treatment. HepG2 and SMMC7721 cells were treated with EPI or <jats:sup>125<\/jats:sup>I or in combination. Colony formation assays were performed to verify the antiproliferation effect. Annexin V\u2013FITC\/PI, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays, and western blotting were performed to analyze cellular apoptosis. Scratch wound healing assays and transwell assays were used to examine migration following different treatments. An isobaric tag for relative and absolute quantitation analysis was used to detect changes in protein expression after <jats:sup>125<\/jats:sup>I treatment, identifying the potential mediating protein cathelicidin (LL\u201037). LL\u201037 protein and WNT pathway\u2010related proteins were detected by western blotting in SMMC7721 and HepG2 cells. Mice were treated with <jats:sup>125<\/jats:sup>I and EPI to evaluate whether EPI enhanced the antitumor effect of <jats:sup>125<\/jats:sup>I.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>EPI promoted <jats:sup>125<\/jats:sup>I\u2010induced apoptosis and reduced the proliferation of HepG2 and SMMC7721 cells. EPI also prevented the migration of HepG2 and SMMC7721 cells. EPI combined with <jats:sup>125<\/jats:sup>I may interfere with the WNT signaling pathway by decreasing LL\u201037 to inhibit HCC development. The antitumor effects of EPI with <jats:sup>125<\/jats:sup>I were verified in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>EPI combined with <jats:sup>125<\/jats:sup>I induced apoptosis and inhibited the proliferation of HCC cells by LL\u201037 downregulating the WNT pathway.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ajco.13873","type":"journal-article","created":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T07:44:41Z","timestamp":1670226281000},"page":"355-364","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Epirubicin may enhance the inhibition of hepatocellular carcinoma induced by iodine\u2010125 seeds through downregulating WNT pathway"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5906-2474","authenticated-orcid":false,"given":"Shilin","family":"Tian","sequence":"first","affiliation":[{"name":"Department of Interventional Medicine 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