{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:51:19Z","timestamp":1790718679798,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,2,21]],"date-time":"2013-02-21T00:00:00Z","timestamp":1361404800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust J Dermatology"],"published-print":{"date-parts":[[2013,5]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>The successful treatment of onychomycosis depends on accurate diagnosis. Conventional diagnostic methods, including direct microscopy and fungal culture, are non\u2010specific, insensitive and time\u2010consuming. Recently, PCR<\/jats:styled-content> has shown promise in improving the diagnosis of onychomycosis. We aimed to evaluate a commercially available PCR<\/jats:styled-content> kit for the in vitro<\/jats:italic> detection of dermatophytes and specifically T<\/jats:styled-content>richophyton\u2009rubrum<\/jats:italic> in nail specimens with suspected onychomycosis, and to compare the detection rates of PCR<\/jats:styled-content> with conventional diagnostic methods.<\/jats:p><\/jats:sec>Methods<\/jats:title>Nail specimens were prospectively collected from patients with clinically suspected onychomycosis. All nail specimens were positive on direct microscopic examination. PCR<\/jats:styled-content> and fungal cultures were administered, and the detection rates of dermatophytes were compared.<\/jats:p><\/jats:sec>Results<\/jats:title>\u2002In all, 107 nail specimens were analysed. The fungal culture was positive in 57 (53%) specimens (38 dermatophytes and 19 non\u2010dermatophytes). PCR<\/jats:styled-content> was positive in 77 (72%) specimens (63 T<\/jats:styled-content>.\u2009rubrum<\/jats:italic> and 14 pan\u2010dermatophyte). A total of 37 specimens (35%) were positive for both fungal culture and PCR<\/jats:styled-content>. PCR<\/jats:styled-content> detected dermatophytes in 39 specimens that were missed by the fungal culture, increasing the diagnosis of dermatophyte\u2010positive specimens by 37%. Five dermatophyte\u2013culture\u2010positive specimens were negative for PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>This study demonstrates that PCR<\/jats:styled-content> increases the sensitivity of detection of dermatophytes in nail specimens. Despite its limitations, the use of PCR<\/jats:styled-content> can complement direct microscopic examination and fungal cultures to aid clinicians in the diagnosis of suspected dermatophytic onychomycosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ajd.12027","type":"journal-article","created":{"date-parts":[[2013,2,21]],"date-time":"2013-02-21T06:53:48Z","timestamp":1361429628000},"page":"105-108","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Complementary role of a polymerase chain reaction test in the diagnosis of onychomycosis"],"prefix":"10.1111","volume":"54","author":[{"given":"Nisha Suyien","family":"Chandran","sequence":"first","affiliation":[{"name":"National Skin Centre Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiun\u2010Yit","family":"Pan","sequence":"additional","affiliation":[{"name":"National Skin Centre Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zacharias AD","family":"Pramono","sequence":"additional","affiliation":[{"name":"National Skin Centre Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiok\u2010Hee","family":"Tan","sequence":"additional","affiliation":[{"name":"Thomson Specialist Skin Centre Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chew\u2010Swee","family":"Seow","sequence":"additional","affiliation":[{"name":"National Skin Centre Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,2,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2000.107754"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.149.s65.4.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780601070107"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"614","DOI":"10.2340\/00015555-0507","article-title":"Laboratory\u2010based survey of dermatophyte infections in Denmark over a 10\u2010year period","volume":"88","author":"Saunte DM","year":"2008","journal-title":"Acta Derm. 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