{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:42:16Z","timestamp":1787240536508,"version":"build-2736575974"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,3,15]],"date-time":"2017-03-15T00:00:00Z","timestamp":1489536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81502715"],"award-info":[{"award-number":["81502715"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["13JC1402302"],"award-info":[{"award-number":["13JC1402302"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust J Dermatology"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      39 and\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      73 are two novel cell surface markers of\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      25\n                      <jats:sup>high<\/jats:sup>\n                      Foxp3\n                      <jats:sup>+<\/jats:sup>\n                      regulatory T\u2010cells (Tregs). Concordant expression of these two ectoenzymes not only discriminate Tregs from other cell populations, but also generates pericellular adenosine, which has been reported to suppress proliferation of activated T effector (Teff) cells. Because it is currently unclear whether human ectoenzymes (\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      39\/\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      73) are involved in the impaired suppressive activity of Tregs in psoriasis, we examined the frequencies and phenotypes of\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      39\/\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      73\u2010expressing Tregs and related receptor adenosine receptor 2A (A\n                      <jats:sub>2A<\/jats:sub>\n                      <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\n                      ) in peripheral blood of patients with different types of psoriasis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Peripheral blood mononuclear cells (\n                      <jats:styled-content style=\"fixed-case\">PMBC<\/jats:styled-content>\n                      ) were prepared from patients with three different types of psoriasis (psoriasis vulgaris, pustular psoriasis and erythrodermic psoriasis).\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      4\n                      <jats:sup>+<\/jats:sup>\n                      cells were separated from\n                      <jats:styled-content style=\"fixed-case\">PBMC<\/jats:styled-content>\n                      by negative selection on midi\n                      <jats:styled-content style=\"fixed-case\">MACS<\/jats:styled-content>\n                      columns, and the frequencies and phenotypes of\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      39 and\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      73 expressing Tregs, and A\n                      <jats:sub>2A<\/jats:sub>\n                      <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\n                      expressing Teff were all determined by flow cytometry analysis. Blood from healthy volunteers served as controls.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The expression of single\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      73\n                      <jats:sup>+<\/jats:sup>\n                      Tregs was markedly reduced (approximately 50%) in psoriasis vulgaris, compared to normal controls. In pustular psoriasis, the mean numbers of\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      39\n                      <jats:sup>+<\/jats:sup>\n                      Tregs and A\n                      <jats:sub>2A<\/jats:sub>\n                      R\n                      <jats:sup>+<\/jats:sup>\n                      Teff was significantly lower than in normal controls. Among three different types of psoriasis,\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      39 expression was strikingly reduced in the blood Treg population of pustular psoriasis patients. Decreased\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      73\n                      <jats:sup>+<\/jats:sup>\n                      Tregs levels were observed in psoriasis vulgaris compared to pustular psoriasis and erythrodermic psoriasis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The differences in the expression of\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      39\n                      <jats:sup>\u2212<\/jats:sup>\n                      and\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      73\n                      <jats:sup>\u2212<\/jats:sup>\n                      Tregs may be a factor in the pathogenesis of psoriasis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ajd.12561","type":"journal-article","created":{"date-parts":[[2017,3,15]],"date-time":"2017-03-15T04:03:09Z","timestamp":1489550589000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Phenotypical analysis of ectoenzymes\n                    <scp>CD<\/scp>\n                    39\/\n                    <scp>CD<\/scp>\n                    73 and adenosine receptor 2A in\n                    <scp>CD<\/scp>\n                    4\n                    <sup>+<\/sup>\n                    <scp>CD<\/scp>\n                    25\n                    <sup>high<\/sup>\n                    Foxp3\n                    <sup>+<\/sup>\n                    regulatory T\u2010cells in psoriasis"],"prefix":"10.1111","volume":"59","author":[{"given":"Ling","family":"Han","sequence":"first","affiliation":[{"name":"Department of Dermatology Huashan Hospital Shanghai China"},{"name":"Department of Dermatology Case Western Reserve University Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideaki","family":"Sugiyama","sequence":"additional","affiliation":[{"name":"Department of Dermatology Case Western Reserve University Cleveland Ohio USA"},{"name":"Department of Dermatology Seirei Yokohama General Hospital Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Huashan Hospital Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kexiang","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Dermatology Huashan Hospital Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Huashan Hospital Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas S","family":"McCormick","sequence":"additional","affiliation":[{"name":"Department of Dermatology Case Western Reserve University Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin D","family":"Cooper","sequence":"additional","affiliation":[{"name":"Department of Dermatology Case Western Reserve University Cleveland Ohio USA"},{"name":"The Murdough Family Center for Psoriasis University Hospital Case Medical Center Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Huashan Hospital Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.155.3.1151"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1026"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1178"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-367-7_20"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20062512"},{"issue":"1","key":"e_1_2_7_7_1","first-page":"S457","article-title":"Adenosine A2A receptor antagonists: blockade of adenosinergic effects and T regulatory cells","volume":"153","author":"Sitkovsky M","year":"2008","journal-title":"Br. 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