{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T12:51:06Z","timestamp":1763643066837,"version":"3.40.5"},"reference-count":14,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,12,16]],"date-time":"2020-12-16T00:00:00Z","timestamp":1608076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust J Dermatology"],"published-print":{"date-parts":[[2021,5]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>The aetiology and pathogenesis of vulvar lichen sclerosus (LS), a chronic inflammatory disease, is not completely clear. It has been found that local cellular immune abnormalities play an important role in the immune mechanism of LS, mainly characterised by abnormal numbers of Langerhans cells in the epidermis and abnormal numbers of dermal T lymphocytes.<\/jats:p><\/jats:sec>Objective<\/jats:title>To evaluate the densities of Langerhans cells and T\u2010lymphocyte subpopulations in vulvar LS.<\/jats:p><\/jats:sec>Methods<\/jats:title>The density of Langerhans cells in the epidermis, and CD3+<\/jats:sup>, CD4+<\/jats:sup> and CD8+<\/jats:sup> T cells in the dermis of seven early\u2010stage and eight late\u2010stage cases of vulvar LS were detected with direct immunofluorescence, and compared with 15 normal controls.<\/jats:p><\/jats:sec>Results<\/jats:title>The density of Langerhans cells in the late\u2010stage group was significantly higher than in the normal group (P<\/jats:italic>\u00a0=\u00a00.001). The densities of CD3+<\/jats:sup>, CD4+<\/jats:sup> and CD8+<\/jats:sup> T lymphocytes in both the early\u2010 and late\u2010stage (deeper dermis) groups were higher than in the normal group (P<\/jats:italic>\u00a0<\u00a00.05). The ratio of CD4+<\/jats:sup>\/CD8+<\/jats:sup>T lymphocytes in the early\u2010stage and normal groups showed no significant difference (P<\/jats:italic>\u00a0=\u00a00.151), while the late\u2010stage (deeper dermis) groups decreased significantly compared with early\u2010stage, late\u2010stage (upper dermis) and normal groups (P<\/jats:italic>\u00a0<\u00a00.001).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The densities of Langerhans cells, CD3+<\/jats:sup>, CD4+<\/jats:sup> and CD8+<\/jats:sup> T cells, and the ratio of CD4+<\/jats:sup>\/CD8+<\/jats:sup>T lymphocytes were different in different stages of LS, which supports the important role of cellular immunity in mechanisms of LS.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ajd.13525","type":"journal-article","created":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T19:36:40Z","timestamp":1608233800000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Study of Langerhans cells and T lymphocytes in vulvar lichen sclerosus lesions"],"prefix":"10.1111","volume":"62","author":[{"given":"Yingqiu","family":"Bao","sequence":"first","affiliation":[{"name":"Department of Dermatology Beijing Hospital National Center of Gerontology Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziyuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology Beijing Hospital National Center of Gerontology Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Beijing Hospital National Center of Gerontology Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Beijing Hospital National Center of Gerontology Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaqi","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Dermatology Beijing Hospital National Center of Gerontology Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kailv","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Dermatology Beijing Hospital National Center of Gerontology Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2077-6626","authenticated-orcid":false,"given":"Jianmin","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Beijing Hospital National Center of Gerontology Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2000.00040.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00005-006-0039-6"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1992.tb14858.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2006.02406.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000051636"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2006.07414.x"},{"key":"e_1_2_6_8_1","first-page":"33","article-title":"Quantity and distribution of Langerhans cells and CD8 + T cells in lesions of alopecia areata","volume":"047","author":"Zhang XT","year":"2014","journal-title":"Chin. 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Med."},{"key":"e_1_2_6_13_1","first-page":"188","article-title":"Detailed studying of Langerhans cells in female genital lichen sclerosus","volume":"22","author":"Kyriakou A","year":"2018","journal-title":"Hippokratia"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-0194"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2011.369"}],"container-title":["Australasian Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ajd.13525","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ajd.13525","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ajd.13525","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T19:08:36Z","timestamp":1692731316000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ajd.13525"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,16]]},"references-count":14,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,5]]}},"alternative-id":["10.1111\/ajd.13525"],"URL":"https:\/\/doi.org\/10.1111\/ajd.13525","archive":["Portico"],"relation":{},"ISSN":["0004-8380","1440-0960"],"issn-type":[{"type":"print","value":"0004-8380"},{"type":"electronic","value":"1440-0960"}],"subject":[],"published":{"date-parts":[[2020,12,16]]},"assertion":[{"value":"2020-07-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}