{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T04:06:25Z","timestamp":1754107585892,"version":"3.40.5"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T00:00:00Z","timestamp":1673827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust J Dermatology"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Actinic keratosis (AK) is considered as precursor lesion of invasive squamous cell carcinoma. Molecular studies on AK are limited because of too small size of the biopsy specimen to obtain enough DNA or RNA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty biopsy cases of AK, followed by second same\u2010sited biopsies, were included. Ten cases were diagnosed with total regression (regression group), while the other 10 were diagnosed with invasive carcinoma (progression group) in the follow\u2010up biopsies. Using digital spatial profiling (DSP) technology, whole\u2010gene expression analysis defined by specific regions of interest was performed for all 20 cases. After the clinicopathological features were assessed, separate and integrated analyses of these features and gene expression patterns were performed using machine\u2010learning technology. All analyses were performed on both lesion keratinocytes (KT) and infiltrated stromal lymphocytes (LC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among the 18,667 genes assessed, 33 and 72 differentially expressed genes (DEGs) between the regression and progression groups were found in KT and LC respectively. The primary genes distinguishing the two groups were <jats:italic>KRT10<\/jats:italic> for KT and <jats:italic>CARD18<\/jats:italic> for LC. Clinicopathological features were weaker in risk stratification of AK progression than the gene expression patterns. Pathways associated with various cancers were upregulated in the progression group of KT, whereas the nucleotide\u2010binding oligomerization domain (NOD)\u2010like receptor signalling pathway was upregulated in the progression of LC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Gene expression patterns were effective for risk stratification of AK progression, and their distinguishing power was higher than that of clinicopathological features.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ajd.13965","type":"journal-article","created":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T15:54:29Z","timestamp":1673884469000},"page":"80-91","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Factors for risk stratification of patients with actinic keratosis using integrated analysis of clinicopathological features and gene expression patterns"],"prefix":"10.1111","volume":"64","author":[{"given":"Han\u2010Na","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Pathology Korea University Guro Hospital  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pathology Korea University Guro Hospital  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeong\u2010An","family":"Gim","sequence":"additional","affiliation":[{"name":"Medical Science Research Center College of Medicine, Korea University Guro Hospital  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoo S.","family":"Baek","sequence":"additional","affiliation":[{"name":"Department of Dermatology Korea University Guro Hospital  Seoul South 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