{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T13:25:12Z","timestamp":1793971512598,"version":"4.8.0"},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust J Dermatology"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Considerable uncertainty exists regarding the prevalence and clinical significance of basement membrane zone (BMZ) anti\u2010IgE antibodies in bullous pemphigoid (BP).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To assess the frequency of linear BMZ anti\u2010IgE antibody deposition by direct immunofluorescence (DIF) and evaluate its association with disease severity and potential diagnostic utility in BP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This prospective cross\u2010sectional analytical study enrolled 50 patients with newly diagnosed BP, confirmed by DIF and serology, and 50 non\u2010BP controls. Associations with clinical disease severity and laboratory parameters were analysed. An exploratory diagnostic performance analysis of anti\u2010IgE positivity was also performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The mean age of the study and control populations was 65.1\u2009\u00b1\u200917 and 58.2\u2009\u00b1\u200918.5\u2009years, respectively. BMZ anti\u2010IgE antibodies were detected in 27\/50 BP patients (54%) and 1\/50 (2%) controls [odds ratio (OR) 57.5, 95% confidence interval (CI), 7.4\u2013449.7;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001]. Anti\u2010IgE positivity was significantly associated with bullous pemphigoid disease area index (BPDAI) total and BPDAI\u2010Erosion\u2010Blister scores, total body surface area, high absolute eosinophil count (AEC), and increased serum IgE. In multivariate logistic regression, elevated AEC and increased serum IgE independently predicted anti\u2010IgE positivity [adjusted\u2010OR (95% CI): 1.004 (1.000\u20131.008);\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.037 and 1.003 (1.000\u20131.005);\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.033, respectively]. Anti\u2010IgE positivity demonstrated 54% sensitivity, 98% specificity and a positive likelihood ratio of 27.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>BMZ anti\u2010IgE antibody was present in over half of the patients with BP and was associated with greater disease severity, eosinophilia and elevated serum IgE. Given its high specificity but modest sensitivity, anti\u2010IgE positivity may serve as a useful adjunctive marker to support, rather than exclude, the diagnosis of BP.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ajd.70211","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T06:34:07Z","timestamp":1789626847000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Detection of Anti\u2010IgE Antibodies Against Basement Membrane Zone in Bullous Pemphigoid by Direct Immunofluorescence: Prevalence, Associations and Significance"],"prefix":"10.1111","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3187-5152","authenticated-orcid":false,"given":"Saurodeep","family":"Basu","sequence":"first","affiliation":[{"name":"Department of Dermatology, Venereology and Leprosy, Kasturba Medical College Manipal Academy of Higher Education  Manipal Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0619-1816","authenticated-orcid":false,"given":"Raghavendra","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Leprosy, Kasturba Medical College Manipal Academy of Higher Education  Manipal Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"e_1_2_13_2_1","volume-title":"StatPearls","author":"Baigrie D.","year":"2026"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2020.02.013"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523\u20101747.2002.19529.x"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(12)61140\u20104"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40257\u2010017\u20100264\u20102"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403\u2010017\u20101789\u20101"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022\u2010202X.2005.23853.x"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2015.02.015"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2009.04.013"},{"issue":"2","key":"e_1_2_13_11_1","first-page":"193","article-title":"Immunopathology of Bullous Pemphigoid. 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