{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:24:31Z","timestamp":1762251871812},"reference-count":9,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,8,14]],"date-time":"2012-08-14T00:00:00Z","timestamp":1344902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>The differential reaction of cytotrophoblast and syncytiotrophoblast towards infection with listeria monocytogenes (LM) is pivotal to its pathogenicity. In this study we tested the cytokine signature upon infection with listeria monocytogenes (LM) in an <jats:italic>in vitro<\/jats:italic> model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>We compared two related trophoblastic cell lines (AC\u20101M32 and ACH1P). The cell line ACH1P showed syncytium formation, whereas AC\u20101M32 did not fuse, as demonstrated with immunfluorescence E\u2010Cadherin staining. In a Multi\u2010Analyte ELISArray we tested for concentrations of TNF\u03b1, IL\u20101\u03b2, IL\u20102, IL\u20104, IL\u20106, IL\u201010, IL\u201012, IL\u201013, IL\u201017A, IL\u20108, MCP1, MIP\u20101a, MIP\u20101b, MDC, Eotaxin, IFN\u03b3, G\u2010CSF, TGF\u03b21 after 8 and 24 hours.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared to unstimulated cells, the syncytial cell line ACH1P showed a significant induction of Interleukin\u20106 (IL\u20106), Monocyte Chemotactic Protein\u20101 (MCP\u20101) and transforming growth factor \u03b2\u20101 (TGF\u03b21). Incubating AC\u20101M32 with LM, however, showed significantly reduced IL\u20106 levels and a massively increased (~300fold) TGF\u03b21 secretion compared to unstimulated controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>A functional anti\u2010LM immune response was only induced by the syncytium\u2010forming cell line ACH1P. Using the two sister cell lines AC\u20101M32 and ACH1P in an <jats:italic>in vitro<\/jats:italic> LM\u2010stimulation assay might facilitate research in the area of placental listeria infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12004","type":"journal-article","created":{"date-parts":[[2012,8,14]],"date-time":"2012-08-14T14:35:16Z","timestamp":1344954916000},"page":"387-391","source":"Crossref","is-referenced-by-count":2,"title":["Trophoblastic Cell Lines <scp>ACH<\/scp>1P and <scp>AC<\/scp>\u20101<scp>M<\/scp>32 React with a Distinctive Cytokine Pattern Toward Listeria Monocytogenes and Show Morphologic Differences"],"prefix":"10.1111","volume":"68","author":[{"given":"Laura","family":"Santoso","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Ludwig\u2010Maximilians University Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Friese","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Ludwig\u2010Maximilians University Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Udo","family":"Jeschke","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Ludwig\u2010Maximilians University Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Scholz","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Ulm University Medical Centre Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,8,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"crossref","first-page":"3947","DOI":"10.4049\/jimmunol.140.11.3947","article-title":"Specific defects in the anti\u2010listerial immune response in discrete regions of the murine uterus and placenta account for susceptibility to infection","volume":"140","author":"Redline RW","year":"1988","journal-title":"J Immunol"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020066"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000732"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.26.021607.090326"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(99)00107-7"},{"key":"e_1_2_5_7_1","first-page":"467","article-title":"Immunohistochemical expression of glycodelin in breast cancer correlates with estrogen\u2010receptor alpha and progesterone\u2010receptor A positivity","volume":"24","author":"Scholz C","year":"2009","journal-title":"Histol Histopathol"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/plac.2000.0559"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2010.06.016"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1059852"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Faji.12004","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.12004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T17:00:11Z","timestamp":1695488411000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.12004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8,14]]},"references-count":9,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.1111\/aji.12004"],"URL":"https:\/\/doi.org\/10.1111\/aji.12004","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8,14]]}}}