{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T04:01:56Z","timestamp":1778126516065,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,9,17]],"date-time":"2012-09-17T00:00:00Z","timestamp":1347840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2013,1]]},"abstract":"Problem<\/jats:title>Expression patterns and regulation of cytosolic pattern recognition receptors (PRR<\/jats:styled-content>) NOD<\/jats:styled-content>\u20101, NOD<\/jats:styled-content>\u20102, RIG<\/jats:styled-content>\u20101, and MDA<\/jats:styled-content>5 have not been elucidated in the human female reproductive tract (FRT<\/jats:styled-content>).<\/jats:p><\/jats:sec>Method of study<\/jats:title>Primary epithelial cells (EC<\/jats:styled-content>) isolated from F<\/jats:styled-content>allopian tube (FT<\/jats:styled-content>), endometrium (EM<\/jats:styled-content>), cervix (C<\/jats:styled-content>x), and ectocervix (E<\/jats:styled-content>cx) were treated with estradiol, poly(I<\/jats:styled-content>:C<\/jats:styled-content>), N<\/jats:styled-content>eisseria gonorrhea<\/jats:italic> (GC<\/jats:styled-content>), and HIV<\/jats:styled-content>\u20101. PRR mRNA<\/jats:styled-content> expressions were analyzed by R<\/jats:styled-content>eal\u2010time RT\u2010PCR<\/jats:styled-content>. Conditioned media were analyzed for IL<\/jats:styled-content>\u20108 by ELISA<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>EC<\/jats:styled-content> from all FRT<\/jats:styled-content> compartments constitutively expressed NOD<\/jats:styled-content>1, NOD<\/jats:styled-content>2, RIG<\/jats:styled-content>\u20101, and MDA<\/jats:styled-content>5 with highest levels expressed by FT<\/jats:styled-content>. Stimulation with poly(I<\/jats:styled-content>:C<\/jats:styled-content>) resulted in upregulation of NOD<\/jats:styled-content>2, RIG<\/jats:styled-content>\u20101, and MDA<\/jats:styled-content>5 in all FRT<\/jats:styled-content> compartments and correlated with increased secretion of IL<\/jats:styled-content>\u20108, whereas estradiol treatment had no effects. Exposure to GC<\/jats:styled-content> and HIV<\/jats:styled-content>\u20101 IIIB<\/jats:styled-content> but not B<\/jats:styled-content>aL<\/jats:styled-content> resulted in selective upregulation of NOD<\/jats:styled-content>2 and MDA<\/jats:styled-content>5.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>PRR<\/jats:styled-content> are expressed throughout the FRT<\/jats:styled-content> and differentially regulated by poly(I<\/jats:styled-content>:C<\/jats:styled-content>), GC<\/jats:styled-content> and HIV<\/jats:styled-content>\u20101.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12019","type":"journal-article","created":{"date-parts":[[2012,9,18]],"date-time":"2012-09-18T03:51:59Z","timestamp":1347940319000},"page":"41-51","source":"Crossref","is-referenced-by-count":43,"title":["Pathogen Recognition in the Human Female Reproductive Tract: Expression of Intracellular Cytosolic Sensors NOD<\/scp>1, NOD<\/scp>2, RIG<\/scp>\u20101, and MDA<\/scp>5 and response to HIV<\/scp>\u20101 and 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