{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T14:49:58Z","timestamp":1789051798133,"version":"build-2803163510"},"reference-count":66,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,3,11]],"date-time":"2013-03-11T00:00:00Z","timestamp":1362960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>Pre\u2010eclampsia (<jats:styled-content style=\"fixed-case\">PE<\/jats:styled-content>), a pregnancy complication of unknown etiology, is a major cause of maternal and fetal mortality and morbidity. Previous studies have described placental genes that are up\u2010regulated in expression in <jats:styled-content style=\"fixed-case\">PE<\/jats:styled-content>, but few studies have addressed placental gene suppression in this syndrome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Gene profiling and quantitative reverse transcription PCR (<jats:styled-content style=\"fixed-case\">qRTPCR<\/jats:styled-content>) analyses were used to identify genes down\u2010regulated in placentas from women with severe preterm PE compared with gestational age\u2010matched normotensive controls with spontaneous preterm birth (<jats:styled-content style=\"fixed-case\">sPTB<\/jats:styled-content>). Western blotting and immunohistochemistry were used to evaluate levels and patterns of cell type\u2013specific protein expression in PE and <jats:styled-content style=\"fixed-case\">sPTB<\/jats:styled-content> group placentas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Levels of macrophage marker [folate receptor (FR)\u2010\u03b2, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>163, and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>68] <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and FR\u2010\u03b2 protein were significantly down\u2010regulated in PE group placentas compared with the <jats:styled-content style=\"fixed-case\">sPTB<\/jats:styled-content> group. Numbers of Hofbauer cells (<jats:styled-content style=\"fixed-case\">HBC<\/jats:styled-content>s, fetal macrophages) and <jats:styled-content style=\"fixed-case\">FR<\/jats:styled-content>\u2010\u03b2 protein in these cells were reduced in <jats:styled-content style=\"fixed-case\">PE<\/jats:styled-content> group placentas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Severe <jats:styled-content style=\"fixed-case\">PE<\/jats:styled-content> is associated with decreased placental expression of <jats:styled-content style=\"fixed-case\">FR<\/jats:styled-content>\u2010\u03b2 and a reduction in the number of <jats:styled-content style=\"fixed-case\">HBC<\/jats:styled-content>s. Reduced placental macrophage function is likely to play a key role in the pathophysiology of <jats:styled-content style=\"fixed-case\">PE<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12112","type":"journal-article","created":{"date-parts":[[2013,3,13]],"date-time":"2013-03-13T03:46:43Z","timestamp":1363146403000},"page":"104-115","source":"Crossref","is-referenced-by-count":65,"title":["Decreased Levels of Folate Receptor\u2010\u03b2 and Reduced Numbers of Fetal Macrophages (<scp>H<\/scp>ofbauer Cells) in Placentas from Pregnancies with Severe Pre\u2010Eclampsia"],"prefix":"10.1111","volume":"70","author":[{"given":"Zhonghua","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Obstetrics\/Gynecology and Reproductive Sciences Yale University School of Medicine New Haven CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina A.","family":"Buhimschi","sequence":"additional","affiliation":[{"name":"Department of 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