{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T08:25:14Z","timestamp":1777537514207,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,7,15]],"date-time":"2013-07-15T00:00:00Z","timestamp":1373846400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>The aim of this study was to identify the characteristics of serum autoantibodies in experimental autoimmune orchitis (<jats:styled-content style=\"fixed-case\">EAO<\/jats:styled-content>) induced by immunization with syngeneic testicular germ cells (<jats:styled-content style=\"fixed-case\">TGC<\/jats:styled-content>) alone in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>The serum autoantibodies were analyzed by enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>), <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>estern blotting, and immunohistochemical staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Serum autoantibody titers against <jats:styled-content style=\"fixed-case\">TGC<\/jats:styled-content> and epididymal spermatozoa (<jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content>) gradually increased with the development of <jats:styled-content style=\"fixed-case\">EAO<\/jats:styled-content>. At the initial stage of <jats:styled-content style=\"fixed-case\">EAO<\/jats:styled-content>, serum autoantibodies reacted with only a few antigens of <jats:styled-content style=\"fixed-case\">TGC<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content>, while in the later stage, the reactions with various antigens of <jats:styled-content style=\"fixed-case\">TGC<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content> occurred. However, the autoantibodies were specific to the testis and epididymis but not to other organs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results indicate that titers and kinds of autoantibodies in <jats:styled-content style=\"fixed-case\">TGC<\/jats:styled-content>\u2010immunized mice increase with <jats:styled-content style=\"fixed-case\">EAO<\/jats:styled-content> development and they are specific to <jats:styled-content style=\"fixed-case\">TGC<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12145","type":"journal-article","created":{"date-parts":[[2013,7,15]],"date-time":"2013-07-15T06:32:37Z","timestamp":1373869957000},"page":"509-517","source":"Crossref","is-referenced-by-count":11,"title":["Serum Autoantibodies in Mice Immunized with Syngeneic Testicular Germ Cells Alone"],"prefix":"10.1111","volume":"70","author":[{"given":"Shuichi","family":"Hirai","sequence":"first","affiliation":[{"name":"Department of Anatomy Tokyo Medical University Shinjuku\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Munekazu","family":"Naito","sequence":"additional","affiliation":[{"name":"Department of Anatomy Tokyo Medical University Shinjuku\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayato","family":"Terayama","sequence":"additional","affiliation":[{"name":"Department of Anatomy Tokyo Medical University Shinjuku\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoyuki","family":"Hatayama","sequence":"additional","affiliation":[{"name":"Department of Anatomy Tokyo Medical University Shinjuku\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Anatomy Tokyo Medical University Shinjuku\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhetaerjiang","family":"Musha","sequence":"additional","affiliation":[{"name":"Department of Anatomy Tokyo Medical University Shinjuku\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Itoh","sequence":"additional","affiliation":[{"name":"Department of Anatomy Tokyo Medical University Shinjuku\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,7,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.1993.tb01192.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.2008.00837.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2010.09.026"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/08916939109004812"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2010.01082.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.111.012989"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00430817"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"2675","DOI":"10.4049\/jimmunol.130.6.2675","article-title":"Immunopathology of murine experimental allergic orchitis","volume":"130","author":"Kohno S","year":"1983","journal-title":"J Immunol"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1989.tb00991.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1991.tb05603.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2007.00556.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2009.03.002"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2007.00790.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00795-009-0469-4"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.3109\/01485019408987766"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00795-012-0587-2"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2010.01137.x"},{"key":"e_1_2_6_19_1","first-page":"111","article-title":"Risk factors for antisperm antibodies in infertile men","volume":"37","author":"Heidenreich A","year":"1997","journal-title":"Am J Reprod Immunol"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1995.tb00927.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-038-0_6"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.1980.tb00594.x"},{"key":"e_1_2_6_23_1","first-page":"555","article-title":"Immune complex orchitis in infertile men. 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