{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:37:35Z","timestamp":1762101455815},"reference-count":60,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p><jats:italic>Clostridium sordellii<\/jats:italic> causes endometrial infections, but little is known regarding host defenses against this pathogen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>We tested the hypothesis that the immunoregulatory lipid prostaglandin (<jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content>) <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content><jats:sub>2<\/jats:sub> suppresses human macrophage clearance of <jats:italic><jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>.\u00a0sordellii<\/jats:italic> through receptor\u2010induced increases in intracellular cyclic adenosine monophosphate (<jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>). The <jats:styled-content style=\"fixed-case\">THP<\/jats:styled-content>\u20101 macrophage cell line was used to quantify <jats:italic>C.\u00a0sordellii<\/jats:italic> phagocytosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>PGE<jats:sub>2<\/jats:sub> increased <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content> levels, activated protein kinase A (<jats:styled-content style=\"fixed-case\">PKA<\/jats:styled-content>), and inhibited the class A scavenger receptor\u2010dependent phagocytosis of <jats:italic>C.\u00a0sordellii<\/jats:italic>. Activation of the EP2 and EP4 receptors increased intracellular <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content> and inhibited phagocytosis, with evidence favoring a more important role for EP4 over <jats:styled-content style=\"fixed-case\">EP<\/jats:styled-content>2. This was supported by <jats:styled-content style=\"fixed-case\">EP<\/jats:styled-content> receptor expression data and the use of pharmacological receptor antagonists. In addition, the <jats:styled-content style=\"fixed-case\">PKA<\/jats:styled-content> isoform <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>I appeared to be more important than <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>II in mediating the suppression of ingestion of <jats:italic>C.\u00a0sordellii<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The endogenous lipid mediator <jats:styled-content style=\"fixed-case\">PGE<\/jats:styled-content><jats:sub>2<\/jats:sub> impairs human innate immune responses against <jats:italic>C.\u00a0sordellii<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12153","type":"journal-article","created":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T08:24:15Z","timestamp":1375345455000},"page":"34-43","source":"Crossref","is-referenced-by-count":23,"title":["EP4 and EP2 Receptor Activation of Protein Kinase A by Prostaglandin E<sub>2<\/sub> Impairs Macrophage Phagocytosis of <i>Clostridium sordellii<\/i>"],"prefix":"10.1111","volume":"71","author":[{"given":"Lisa M.","family":"Rogers","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases Department of Internal Medicine University of Michigan Health System Ann Arbor MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tennille","family":"Thelen","sequence":"additional","affiliation":[{"name":"Department of Immunology University of Washington School of Medicine Seattle WA USA"},{"name":"Immunology Program Benaroya Research Institute at Virginia Mason Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krystle","family":"Fordyce","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Internal Medicine University of Michigan Health System Ann Arbor MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilie","family":"Bourdonnay","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine Department of Internal Medicine University of Michigan Health System Ann Arbor MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Casey","family":"Lewis","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine Department of Internal Medicine University of Michigan Health System Ann Arbor MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Biological, Chemical Sciences and Engineering Jiaxing University Jiaxing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junyong","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Biological, Chemical Sciences and Engineering Jiaxing University Jiaxing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingli","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Biological, Chemical Sciences and Engineering Jiaxing University Jiaxing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos H.","family":"Serezani","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Indiana University School of Medicine Indianapolis IN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Peters\u2010Golden","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine Department of Internal Medicine University of Michigan Health System Ann Arbor MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Aronoff","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Internal Medicine University of Michigan Health System Ann Arbor MI USA"},{"name":"Department of Microbiology and Immunology University of Michigan Medical School Ann Arbor MI USA"},{"name":"Reproductive Sciences Program University of Michigan Medical School Ann Arbor MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1086\/508866"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11908-011-0207-7"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2007.00235.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043635"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2011.12.019"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1000989"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.12.8222"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plefa.2003.04.009"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.2741\/2151"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986709789578178"},{"issue":"1","key":"e_1_2_6_12_1","first-page":"65","article-title":"A postgenomic integrated view of prostaglandins in reproduction: implications for other body systems","volume":"59","author":"Fortier MA","year":"2008","journal-title":"J Physiol Pharmacol"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R600038200"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2011.75"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1101029"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.1.559"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.8.5499"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1000838"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2002448"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(73)90090-7"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1159\/000183426"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/14767050701830514"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/ic9023629"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(93)90400-2"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.01-0739fje"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2007.10.004"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1388-1981(99)00164-X"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.9540"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.7.4689"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M204810200"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.2.1092"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2008-0091TR"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.174813"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.187815"},{"key":"e_1_2_6_36_1","unstructured":"WHO: Maternal mortality in 2005: estimates developed by WHO UNICEF UNFPA and the World Bank(http:\/\/www.who.int\/whosis\/mme_2005.pdf)2007."},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijgo.2011.05.027"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2007.10.006"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(09)70172-0"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2010.00836.x"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2011.01083.x"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir325"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir902"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.4997\/JRCPE.2011.411"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2008.05.001"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2008.01.062"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1200821"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00448-12"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.9.5204-5215.2004"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26268-0"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2004.12.024"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.090551"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2002.01718.x"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(88)90240-7"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(90)90138-H"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1986.tb00075.x"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(91)90332-8"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2005.07.026"},{"key":"e_1_2_6_59_1","first-page":"1530","article-title":"Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester","volume":"42","author":"Tsuchiya S","year":"1982","journal-title":"Cancer Res"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910260208"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1202932"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Faji.12153","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.12153","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T15:30:51Z","timestamp":1695483051000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.12153"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8]]},"references-count":60,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["10.1111\/aji.12153"],"URL":"https:\/\/doi.org\/10.1111\/aji.12153","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8]]}}}