{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T05:09:50Z","timestamp":1697432990980},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,9,12]],"date-time":"2013-09-12T00:00:00Z","timestamp":1378944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>The receptor for the globular head of human C1q (<jats:styled-content style=\"fixed-case\">gC<\/jats:styled-content>1qR) predominantly localizes to the mitochondrial matrix. <jats:styled-content style=\"fixed-case\">gC<\/jats:styled-content>1qR mediates many biological responses, including growth perturbations, morphological abnormalities and the initiation of apoptosis. The purpose of this study was to investigate the relationship between <jats:styled-content style=\"fixed-case\">gC<\/jats:styled-content>1qR expression, mitochondrial dysfunction and the regulation of apoptosis in human extravillous cytotrophoblast (EVCT)\u2010derived transformed cell lines (HTR\u20108\/SVneo and HPT\u20108).<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">gC<\/jats:styled-content>1qR expression was examined in human placental villi using real\u2010time <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> and Western blot analysis. The apoptotic death of HTR\u20108\/SVneo and HPT\u20108 cells was assessed using flow cytometric analysis. Mitochondrial function was assessed via ROS generation, the amount of cytosolic Ca<jats:sup>2+<\/jats:sup> and changes in the mitochondrial membrane potential (\u0394\u03c8m).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expression of the <jats:styled-content style=\"fixed-case\">gC<\/jats:styled-content>1qR gene was significantly increased in spontaneous abortion samples relative to induced abortion samples. HTR\u20108\/SVneo and HPT\u20108 cells transfected with a <jats:styled-content style=\"fixed-case\">gC<\/jats:styled-content>1qR vector showed upregulation of cellular apoptosis and mitochondrial dysfunction, interestingly, which were abrogated by the addition of metformin. Metformin may protect mitochondrial function.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These data support a mechanism whereby <jats:styled-content style=\"fixed-case\">gC<\/jats:styled-content>1qR induces apoptosis through mitochondria\u2010dependent pathways in human EVCT\u2010derived transformed cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12160","type":"journal-article","created":{"date-parts":[[2013,9,12]],"date-time":"2013-09-12T12:22:39Z","timestamp":1378988559000},"page":"73-85","source":"Crossref","is-referenced-by-count":2,"title":["The Globular Heads of the <scp>C<\/scp>1q Receptor Regulate Apoptosis in Human Extravillous Cytotrophoblast\u2010derived Transformed Cells via a Mitochondria\u2010dependent Pathway"],"prefix":"10.1111","volume":"71","author":[{"given":"Ying","family":"Wang","sequence":"first","affiliation":[{"name":"Clinical Laboratory Harbin Medical University Cancer Hospital Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu\u2010yu","family":"Guo","sequence":"additional","affiliation":[{"name":"National Centre for Epidemiology and Population Health The Australian National University Canberra Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping\u2010qing","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory State Key Laboratory of Reproductive Medicine Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010man","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory State Key Laboratory of Reproductive Medicine Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University Nanjing 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