{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T10:55:06Z","timestamp":1776423306140,"version":"3.51.2"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T00:00:00Z","timestamp":1381536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>Endocervical epithelial cells express pattern recognition receptors (<jats:styled-content style=\"fixed-case\">PRR<\/jats:styled-content>s) that aid in innate immune responses. Mechanisms regulating signaling of <jats:styled-content style=\"fixed-case\">PRR<\/jats:styled-content>s are poorly understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods of Study<\/jats:title><jats:p>Endocervical cells (End1\/E6E7) were treated with ligands of TLR9 and RIG\u2010I once or after pre\u2010stimulation with same ligand. Cytokine responses were determined by ELISA. Differential gene expression was analyzed by microarray. Differentially expressed genes were validated by qPCR \/Western blot. Role of let\u20107f was studied by inhibition and over\u2010expression studies using commercial inhibitors and let\u20107f encoding plasmids, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Single stimulation of cells with <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>9 ligand, but not <jats:styled-content style=\"fixed-case\">RIG<\/jats:styled-content>\u2010I ligand, induced tolerance to subsequent challenge to the same ligand. Stimulation with <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>9 decreased let\u20107f and increased its target Blimp\u20101. Conversely, <jats:styled-content style=\"fixed-case\">RIG<\/jats:styled-content>\u2010I stimulation increased let\u20107f and decreased Blimp\u20101 expression. Inhibition and over\u2010expression revealed let\u20107f is involved in induction of immune tolerance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We identify let\u20107f as a novel regulator of <jats:styled-content style=\"fixed-case\">PRR<\/jats:styled-content> signaling in endocervical cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12165","type":"journal-article","created":{"date-parts":[[2014,1,10]],"date-time":"2014-01-10T14:24:04Z","timestamp":1389363844000},"page":"137-153","source":"Crossref","is-referenced-by-count":19,"title":["Micro<scp>RNA<\/scp> Let\u20107f: A Novel Regulator of Innate Immune Response in Human Endocervical Cells"],"prefix":"10.1111","volume":"71","author":[{"given":"Ameya","family":"Sathe","sequence":"first","affiliation":[{"name":"Division of Molecular Immunology and Microbiology (MIM) National Institute for Research in Reproductive Health (ICMR) Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mandar S.","family":"Patgaonkar","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Tata Institute for Fundamental Research Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tahir","family":"Bashir","sequence":"additional","affiliation":[{"name":"Division of Molecular Immunology and Microbiology (MIM) National Institute for Research in Reproductive Health (ICMR) Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kudumula Venkata Rami","family":"Reddy","sequence":"additional","affiliation":[{"name":"Division of Molecular Immunology and Microbiology (MIM) National Institute for Research in Reproductive Health (ICMR) Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,10,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1863"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/653212"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1391"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2008.12.004"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2012.55"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20081370"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1500"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1107114108"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0703321"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.8.5082"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2011.19"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2957"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E12-07-0519"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02871"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-020711-075013"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2419-10.2010"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.01.014"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1593\/tlo.09151"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.94"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M702633200"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-11-19"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.20.7065-7077.2001"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1105852108"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.07.031"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2011.00970.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v20.i5.30"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00046-08"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod57.4.847"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-3231.2008.01740.x"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.3.1733"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.044255-0"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.26.021607.090241"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.091291"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901543"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.9.5556"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.7.2592-2603.2000"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05836"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20110658"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-0561"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.2119\/molmed.2010.00225"},{"key":"e_1_2_6_43_1","first-page":"121","volume-title":"Cytokines in Health and Disease","author":"Kunkel SL","year":"1997"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.58.030196.001345"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.22719.x"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.2.671"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.20.10925"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2008.07.007"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Faji.12165","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.12165","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T04:14:03Z","timestamp":1696479243000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.12165"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10,12]]},"references-count":47,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,2]]}},"alternative-id":["10.1111\/aji.12165"],"URL":"https:\/\/doi.org\/10.1111\/aji.12165","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,12]]}}}