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Foetal membranes were also obtained from a distal site (<jats:styled-content style=\"fixed-case\">DS<\/jats:styled-content>) and supracervical site (overlying the cervix; <jats:styled-content style=\"fixed-case\">SCS<\/jats:styled-content>). <jats:styled-content style=\"fixed-case\">SLIT<\/jats:styled-content>3 gene silencing was achieved using si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> in primary amnion and myometrial cells. Pro\u2010inflammatory and pro\u2010labour mediators were evaluated by q<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> ELISA<\/jats:styled-content> and gelatin zymography.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">SLIT<\/jats:styled-content>3 expression was greater in foetal membranes from the <jats:styled-content style=\"fixed-case\">SCS<\/jats:styled-content> compared with <jats:styled-content style=\"fixed-case\">DS<\/jats:styled-content> and in myometrium after term spontaneous labour onset. <jats:styled-content style=\"fixed-case\">SLIT<\/jats:styled-content>3 si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> in primary amnion and myometrial cells decreased <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2\u2010induced pro\u2010inflammatory cytokine gene expression and release (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108) and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 gene expression and release. In amnion cells, <jats:styled-content style=\"fixed-case\">SLIT<\/jats:styled-content>3 si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> knockdown decreased <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2\u2010induced <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\u20102 expression and prostaglandin <jats:styled-content style=\"fixed-case\">PGE<\/jats:styled-content><jats:sub>2<\/jats:sub> release. There was no effect of <jats:styled-content style=\"fixed-case\">SLIT<\/jats:styled-content>3 si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> on <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2\u2010induced <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03ba<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> transcriptional activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results demonstrate that <jats:styled-content style=\"fixed-case\">SLIT<\/jats:styled-content>3 is increased with labour, and both our amnion and our myometrial studies describe a pro\u2010inflammatory effect of <jats:styled-content style=\"fixed-case\">SLIT<\/jats:styled-content>3 in these tissues.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12181","type":"journal-article","created":{"date-parts":[[2013,11,29]],"date-time":"2013-11-29T12:13:02Z","timestamp":1385727182000},"page":"297-311","source":"Crossref","is-referenced-by-count":19,"title":["<scp>SLIT<\/scp>3 is Increased in Supracervical Human Foetal Membranes and in Labouring Myometrium and Regulates Pro\u2010Inflammatory Mediators"],"prefix":"10.1111","volume":"71","author":[{"given":"Ratana","family":"Lim","sequence":"first","affiliation":[{"name":"Mercy Perinatal Research Centre Mercy Hospital for Women Heidelberg Vic. 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