{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:43:18Z","timestamp":1791420198245,"version":"4.3.3"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,1,10]],"date-time":"2014-01-10T00:00:00Z","timestamp":1389312000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2014,3]]},"abstract":"\n Problem<\/jats:title>\n \n Knowledge of the mucosal immune cell composition of the human female genital tract is important for understanding susceptibility to\n HIV<\/jats:styled-content>\n \u20101.\n <\/jats:p>\n <\/jats:sec>\n \n Method of study<\/jats:title>\n We developed an optimized procedure for multicolor flow cytometry analysis of immune cells from human cervix to characterize all major immune cell subsets in the endocervix and ectocervix.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Half of tissue hematopoietic cells were\n CD<\/jats:styled-content>\n 14\n +<\/jats:sup>\n , many of which were macrophages and about a third were\n CD<\/jats:styled-content>\n 11c\n +<\/jats:sup>\n , most of which were\n CD<\/jats:styled-content>\n 103\n \u2212<\/jats:sup>\n \u00a0\n CD<\/jats:styled-content>\n 11b\n +<\/jats:sup>\n \u00a0\n CX<\/jats:styled-content>\n 3\n CR<\/jats:styled-content>\n 1\n +<\/jats:sup>\n \u00a0\n DC<\/jats:styled-content>\n \u2010\n SIGN<\/jats:styled-content>\n +<\/jats:sup>\n dendritic cells (\n DC<\/jats:styled-content>\n s). The other dominant population were T cells, with more\n CD<\/jats:styled-content>\n 8 than\n CD<\/jats:styled-content>\n 4 cells. T cells (both\n CD<\/jats:styled-content>\n 8 and\n CD<\/jats:styled-content>\n 4) and B cells were more abundant in the ectocervix than endocervix of pre\u2010menopausal women; however,\n CD<\/jats:styled-content>\n 8\n +<\/jats:sup>\n T cell and B cell numbers declined in the ectocervix after menopause, while\n CD<\/jats:styled-content>\n 4 T cell counts remained higher. B,\n NK<\/jats:styled-content>\n and conventional myeloid and plasmocytoid\n DC<\/jats:styled-content>\n s each were a few percent of tissue hematopoietic cells. Although the ectocervix had more\n HIV<\/jats:styled-content>\n \u2010susceptible\n CD<\/jats:styled-content>\n 4\n +<\/jats:sup>\n T cells, polarized endocervical explants supported\n HIV<\/jats:styled-content>\n replication significantly better.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Due to their abundance in the genital tract,\n CX<\/jats:styled-content>\n 3\n CR<\/jats:styled-content>\n 1\n +<\/jats:sup>\n \u00a0\n DC<\/jats:styled-content>\n \u2010\n SIGN<\/jats:styled-content>\n +<\/jats:sup>\n DC<\/jats:styled-content>\n s might be important in\n HIV<\/jats:styled-content>\n transmission. Our data also suggest that the columnar epithelium of the upper genital tract might be a preferential site for\n HIV<\/jats:styled-content>\n transmission.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/aji.12198","type":"journal-article","created":{"date-parts":[[2014,1,10]],"date-time":"2014-01-10T12:01:45Z","timestamp":1389355305000},"page":"252-264","source":"Crossref","is-referenced-by-count":100,"title":["Distribution of Immune Cells in the Human Cervix and Implications for\n HIV<\/scp>\n Transmission"],"prefix":"10.1111","volume":"71","author":[{"given":"Radiana T.","family":"Trifonova","sequence":"first","affiliation":[{"name":"Program in Cellular and Molecular Medicine Boston Children's Hospital Harvard Medical School Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judy","family":"Lieberman","sequence":"additional","affiliation":[{"name":"Program in Cellular and Molecular Medicine Boston Children's Hospital Harvard Medical School Boston MA 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