{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T10:34:41Z","timestamp":1780914881826,"version":"3.54.1"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,2,18]],"date-time":"2014-02-18T00:00:00Z","timestamp":1392681600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>To determine the effect of collagen from maternal\u2013fetal interface on decidual natural killer cell (d<jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content>) function.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Decidual and villous samples were collected from normal pregnancy and miscarriage. The phenotype and cytokine production were analyzed, respectively, by flow cytometry and enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>). Co\u2010culture was established to investigate the effect of trophoblasts and decidual stromal cells (<jats:styled-content style=\"fixed-case\">DSC<\/jats:styled-content>s) on d<jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content>s.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Maternal\u2013fetal interface of normal pregnancy showed higher collagen and <jats:styled-content style=\"fixed-case\">LAIR<\/jats:styled-content>\u20101 expression than that of miscarriage. Co\u2010culture of d<jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content>s with <jats:styled-content style=\"fixed-case\">HTR<\/jats:styled-content>\u20108\/<jats:styled-content style=\"fixed-case\">DSC<\/jats:styled-content>s up\u2010regulated <jats:styled-content style=\"fixed-case\">LAIR<\/jats:styled-content>\u20101 on d<jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content>s that could be attenuated by pre\u2010treatment with <jats:styled-content style=\"fixed-case\">LAIR<\/jats:styled-content>\u20102, a competitive inhibitor of <jats:styled-content style=\"fixed-case\">LAIR<\/jats:styled-content>\u20101. Collagen down\u2010regulated expression of cell surface receptor activity and intracellular perforin, while it up\u2010regulated expression of suppressive receptor on d<jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content>s. Co\u2010culture of d<jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content>s with <jats:styled-content style=\"fixed-case\">HTR<\/jats:styled-content>\u20108\/<jats:styled-content style=\"fixed-case\">DSC<\/jats:styled-content>s decreased perforin expression and <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>h1\u2010type cytokines production by d<jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content>s, which could be abrogated by <jats:styled-content style=\"fixed-case\">LAIR<\/jats:styled-content>\u20102. In addition, silence of collagen in <jats:styled-content style=\"fixed-case\">HTR<\/jats:styled-content>\u20108\/<jats:styled-content style=\"fixed-case\">DSC<\/jats:styled-content>s by sh<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> significantly attenuated regulation on d<jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content>s.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Trophoblasts and <jats:styled-content style=\"fixed-case\">DSC<\/jats:styled-content>s regulate decidual <jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content> cell functions via secreting collagen, which is involved in the maintenance of human pregnancy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12211","type":"journal-article","created":{"date-parts":[[2014,2,18]],"date-time":"2014-02-18T12:55:55Z","timestamp":1392728155000},"page":"368-378","source":"Crossref","is-referenced-by-count":33,"title":["Trophoblasts and Decidual Stromal Cells Regulate Decidual <scp>NK<\/scp> Cell Functions Via Interaction between Collagen and <scp>LAIR<\/scp>\u20101"],"prefix":"10.1111","volume":"71","author":[{"given":"Qiang","family":"Fu","sequence":"first","affiliation":[{"name":"Laboratory for Reproductive Immunology Hospital and Institute of Obstetrics &amp; 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