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However, little is known about the involvement and regulation of micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (miRs) in genital <jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We analyzed miRs in the genital tract (<jats:styled-content style=\"fixed-case\">GT<\/jats:styled-content>) following <jats:italic>C.\u00a0muridarum<\/jats:italic> (murine strain of <jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content>) challenge of wild type (<jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T\u2010cell deficient (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>\u2212\/\u2212<\/jats:sup>) C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 mice at days 6 and 12 post\u2010challenge.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At day 6, miRs significantly downregulated in the lower <jats:styled-content style=\"fixed-case\">GT<\/jats:styled-content> were miR\u2010125b\u20105p, \u201016, \u2010214, \u201023b, \u2010135a, \u2010182, \u2010183, \u201030c, and \u201030e while \u2010146 and \u2010451 were significantly upregulated, profiles not exhibited at day 12 post\u2010bacterial challenge. Significant differences in miR\u2010125b\u20105p (+5.06\u2010fold change), \u2010135a (+4.9), \u2010183 (+7.9), and \u2010182 (+3.2) were observed in <jats:italic>C.\u00a0muridarum<\/jats:italic>\u2010infected <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>\u2212\/\u2212<\/jats:sup> compared to <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content> mice. <jats:italic>In silico<\/jats:italic> prediction and mass spectrometry revealed regulation of miR\u2010135a and \u2010182 and associated proteins, <jats:italic>that is<\/jats:italic>, heat\u2010shock protein B1 and alpha\u20102<jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>\u2010glycoprotein.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study provides evidence on regulation of miRs following genital chlamydial infection suggesting a role in pathogenesis and host immunity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12281","type":"journal-article","created":{"date-parts":[[2014,6,28]],"date-time":"2014-06-28T07:01:30Z","timestamp":1403938890000},"page":"126-140","source":"Crossref","is-referenced-by-count":27,"title":["<i>Chlamydia muridarum<\/i> Infection Associated Host Micro<scp>RNA<\/scp>s in the Murine Genital Tract and Contribution to Generation of Host Immune Response"],"prefix":"10.1111","volume":"73","author":[{"given":"Rishein","family":"Gupta","sequence":"first","affiliation":[{"name":"South Texas Center for Emerging Infectious Diseases and Center of Excellence in Infection Genomics University of Texas at San Antonio San Antonio TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanvi","family":"Arkatkar","sequence":"additional","affiliation":[{"name":"South Texas Center for Emerging Infectious Diseases and Center of Excellence in Infection Genomics University of Texas at San Antonio San Antonio TX 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