{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T19:47:59Z","timestamp":1785008879323,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>Aberrant trophoblast invasion has been associated with human intrauterine growth restriction (<jats:styled-content style=\"fixed-case\">IUGR<\/jats:styled-content>) and preeclampsia (<jats:styled-content style=\"fixed-case\">PE<\/jats:styled-content>). Our objective was to determine placenta growth factor (PlGF)\u2010mediated regulation of cell invasion in trophoblast cells with reduced mammalian target of Rapamycin (<jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content>) signaling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>First trimester SW 71 trophoblast cells were subjected to invasion assays with the following conditions: 10% FBS, 10% FBS with Rapamycin, and 10% FBS with Rapamycin and PlGF. <jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content> siRNA was also done in these cells. Western blots were performed on cell lysates with antibodies against phospho\u2010 and total <jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content>, 70\u2010kDa ribosomal protein kinase I (p70), 4EBP1, extracellular regulated kinase (ERK), and phosphatidylinositol\u20103 kinase (<jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared to controls, trophoblast cells showed: (i) a 33% decrease in invasion following Rapamycin treatment, (ii) protection from decreased invasion following Rapamycin and PlGF treatment, (iii) a 31% decrease in <jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content> phosphorylation with Rapamycin, (iv) increased phosphorylation of p70 (43%) with Rapamycin and PlGF, and (v) a 76% decrease in invasion following <jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content> depletion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We conclude that first trimester trophoblast invasion is functionally decreased when phosphorylation of <jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content> is prevented and this decrease is recovered with the addition of PlGF. Mechanistically, this recovery involves the phosphorylation of p70 independent of <jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12327","type":"journal-article","created":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T09:45:39Z","timestamp":1412156739000},"page":"330-340","source":"Crossref","is-referenced-by-count":36,"title":["Placenta Growth Factor Induces Invasion and Activates p70 during Rapamycin Treatment in Trophoblast Cells"],"prefix":"10.1111","volume":"73","author":[{"given":"Allison","family":"Knuth","sequence":"first","affiliation":[{"name":"Department of Physiology and Developmental Biology Brigham Young University Provo UT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Physiology and Developmental Biology Brigham Young University Provo UT 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