{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:36:47Z","timestamp":1790422607746,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,4,10]],"date-time":"2015-04-10T00:00:00Z","timestamp":1428624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Key Technology R & D Program of China","award":["2006BAI03B12"],"award-info":[{"award-number":["2006BAI03B12"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>In our previous study on adult male mice, we had identified one immunodominant epitope in <jats:styled-content style=\"fixed-case\">hE<\/jats:styled-content>ppin and three epitopes in <jats:styled-content style=\"fixed-case\">hFSHR<\/jats:styled-content> that caused fertility inhibition. But it only demonstrated a moderate inhibitory effect on fertility, and the antifertility effect was unsatisfactory.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Based on the protein prime\u2013peptide boost inoculation modalities, we further investigated whether the antifertility capacity could be enhanced by a combined immunization with the two antigens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results displayed a enhanced suppressed fertility (F2<jats:styled-content style=\"fixed-case\">EP<\/jats:styled-content>2C 6.67%) in male mice similar to that seen after four separate administrations of the two proteins (F12E\u20104 5%). The most likely mechanism by which this antifertility efficacy was achieved was probably through the production of antibodies that led not only to impairment of spermatogenesis but also to inhibition of sperm motility. Moreover, this treatment also induced high concentrations of neutralizing antibodies which were secreted into the lumen of the epididymis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Thus, a combination immunization with <jats:styled-content style=\"fixed-case\">hFSHR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">hE<\/jats:styled-content>ppin enhanced the contraceptive effects and may provide a better means of immunocontraception.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12381","type":"journal-article","created":{"date-parts":[[2015,4,10]],"date-time":"2015-04-10T23:37:42Z","timestamp":1428709062000},"page":"156-168","source":"Crossref","is-referenced-by-count":8,"title":["Enhanced Suppression of Fertility Can be Achieved by Priming with FSHR and Eppin and Further Boosting with Their B\u2010cell Epitope Peptides"],"prefix":"10.1111","volume":"74","author":[{"given":"Ping","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Southwest Hospital Third Military Medical University Chongqing China"},{"name":"Institute of Immunology Third Military University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"He","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Southwest Hospital Third Military Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzhang","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Immunology Third Military University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengqiong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Southwest Hospital Third Military Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyang","family":"He","sequence":"additional","affiliation":[{"name":"Institute of Immunology Third Military University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Ni","sequence":"additional","affiliation":[{"name":"Institute of Immunology Third Military University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Immunology Third Military University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Immunology Third Military University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zigang","family":"Shen","sequence":"additional","affiliation":[{"name":"Institute of Immunology Third Military University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolan","family":"Fu","sequence":"additional","affiliation":[{"name":"Institute of Immunology Third Military University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqing","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Southwest Hospital Third Military Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jintao","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Immunology Third Military University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,4,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.01.089"},{"key":"e_1_2_7_3_1","first-page":"83","article-title":"Peptide vaccines in immunocontraception","volume":"6","author":"Ferro VA","year":"2004","journal-title":"Curr Opin Mol Ther"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5451.321"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dei256"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200565050-00002"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0188-4409(01)00333-2"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2005.00285.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0220151"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.102.003699"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.036483"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.031567"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1099743"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.11.025"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/09513590903015536"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02788751"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a138198"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1993.tb00385.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0540103"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2005.11.009"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.3.1580"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0890397"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0083-6729(02)64008-7"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv.21.5.0409"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1210\/rp.57.1.149"},{"key":"e_1_2_7_26_1","doi-asserted-by":"crossref","first-page":"3399","DOI":"10.1210\/jcem.87.7.8681","article-title":"Variability in sperm suppression during testosterone administration to adult monkeys is related to follicle stimulating hormone suppression and not to intratesticular androgens","volume":"87","author":"Narula A","year":"2002","journal-title":"J Clin Endocrinol Metab"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20303"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(01)00462-0"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.107.060194"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2005.12.039"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.108.072942"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.03.033"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.01.041"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Faji.12381","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.12381","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T16:46:47Z","timestamp":1695574007000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.12381"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,10]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.1111\/aji.12381"],"URL":"https:\/\/doi.org\/10.1111\/aji.12381","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,10]]}}}