{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T06:23:24Z","timestamp":1759040604635},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,5,24]],"date-time":"2015-05-24T00:00:00Z","timestamp":1432425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Juravinski Cancer Center Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200 tolerance\u2010signaling molecule that is expressed by a wide variety of tissues, including placental trophoblast and epithelial tumor cells, lacks an intracytoplasmic tail and must act by binding to <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200 receptors that have a limited expression on lymphomyeloid cells. This binding can inhibit inflammation and <jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content> cells, promote macrophage secretion of indoleamine\u20102,3 dioxygenase (<jats:styled-content style=\"fixed-case\">IDO<\/jats:styled-content>), and promote generation of Treg cells. Recently, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200R1 was reported on human first trimester placental villous trophoblast cells. <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200R1 has not been described on malignant tumor cells. As malignant tumor cells exhibit a number of characteristics of trophoblast, is <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200R1 expressed?<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Affinity\u2010purified rabbit polyclonal antibodies to <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200 and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200R1 were used to immunostain tissue blocks available from cases in a previous cross\u2010sectional study of Stage 1\u2010<jats:styled-content style=\"fixed-case\">IIIA<\/jats:styled-content> human breast cancer cases and term placental trophoblast.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Affinity\u2010purified anti\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200R1 stained primary breast cancer cells and term placental villous trophoblasts. Tumor cells were also stained by anti\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200 as in a previous study (correlation <jats:italic>P<\/jats:italic>\u00a0=\u00a00.0042), but <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200R1 and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200 were not correlated. Presence or absence of strong <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200 expression in the tumor did not correlate with metastasis, and a similar result was obtained with <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200R1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This is the first report of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200R1 expression by human epithelial tumor cells, and specifically, early\u2010stage human breast cancer cells. It is also the first report of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200R1 expression by term placental villous trophoblasts. The potential biological significance of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200R1 expression in non\u2010hematopoietic cells is discussed.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12403","type":"journal-article","created":{"date-parts":[[2015,5,25]],"date-time":"2015-05-25T14:11:46Z","timestamp":1432563106000},"page":"387-391","source":"Crossref","is-referenced-by-count":4,"title":["The Receptor for the <scp>CD<\/scp>200 Tolerance\u2010Signaling Molecule Associated with Successful Pregnancy is Expressed by Early\u2010Stage Breast Cancer Cells in 80% of Patients and by Term Placental Trophoblasts"],"prefix":"10.1111","volume":"74","author":[{"given":"David A.","family":"Clark","sequence":"first","affiliation":[{"name":"Department of Medicine McMaster University Health Sciences Center Rm 3H1E Hamilton ON Canada"},{"name":"Department of Molecular Medicine &amp; Pathology McMaster University Hamilton ON Canada"},{"name":"Affiliate Scientist Toronto General Research Institute Toronto ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sukhbinder","family":"Dhesy\u2010Thind","sequence":"additional","affiliation":[{"name":"Department of Oncology McMaster University and Juravinski Cancer Center Hamilton ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge L.","family":"Arredondo","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine &amp; Pathology McMaster University Hamilton ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter M.","family":"Ellis","sequence":"additional","affiliation":[{"name":"Department of Oncology McMaster University and Juravinski Cancer Center Hamilton ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer A.","family":"Ramsay","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine &amp; Pathology McMaster University Hamilton ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,5,24]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.6.3034"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/9781608057337113010026"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2004.00192.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/7.2.185"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2013.03.006"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2012.07.007"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0897.2003.00086.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/aogs.12476"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-148X.2012.01025.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1924"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-007-0429-6"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.11.103"},{"key":"e_1_2_5_14_1","unstructured":"TalebianF:CD200\u2010CD200R interaction in tumor immunity. 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