{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T20:36:17Z","timestamp":1773866177984,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,7,28]],"date-time":"2015-07-28T00:00:00Z","timestamp":1438041600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>Ovarian hyperstimulation syndrome (<jats:styled-content style=\"fixed-case\">OHSS<\/jats:styled-content>) is similar to vascular leak syndrome (<jats:styled-content style=\"fixed-case\">VLS<\/jats:styled-content>), which may be attributable to the massive increase in systemic inflammatory cytokines. The hyperstimulated human ovaries were demonstrated to contain interleukin (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>)\u20102, which, in turn, was suggested to activate the systemic inflammatory response characteristic of <jats:styled-content style=\"fixed-case\">OHSS<\/jats:styled-content>. As the source of follicular fluid <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102 is still unclear, in the present study, we sought to validate the presence of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression in human luteinized granulosa cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of Study<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102 nuclear expression was detected using real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and immunofluorescence staining of human luteinized granulosa cell from 6 patients undergoing <jats:italic>in\u00a0vitro<\/jats:italic> fertilization treatment. Calretinin immunofluorescence staining was used as a marker of granulosa cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102\u2010positive immunofluorescence staining was detected within nuclei of granulosa cells, together with positive stain for calretinin, confirming the presence of granulosa cell. Moreover, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102 gene expression was demonstrated in luteinized granulosa cells by real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In the present study, we provided firm evidence for the <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102 production by human luteinized granulosa cells, as demonstrated by the presence of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression in luteinized granulosa cells. Further studies are justified in an attempt to clarify the regulation and the cause\u2010and\u2010effect relationship between <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102 production by the hyperstimulated ovaries and <jats:styled-content style=\"fixed-case\">OHSS<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12416","type":"journal-article","created":{"date-parts":[[2015,7,29]],"date-time":"2015-07-29T05:07:38Z","timestamp":1438146458000},"page":"392-397","source":"Crossref","is-referenced-by-count":7,"title":["Interleukin\u20102 Production by Cultured Human Granulosa Cells"],"prefix":"10.1111","volume":"74","author":[{"given":"Raoul","family":"Orvieto","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Chaim Sheba Medical Center (Tel Hashomer) Ramat Gan Israel"},{"name":"Sackler Faculty of Medicine Tel Aviv University Tel Aviv Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Dratviman\u2010Storobinsky","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Chaim Sheba Medical Center (Tel Hashomer) Ramat Gan Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoram","family":"Cohen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Chaim Sheba Medical Center (Tel Hashomer) Ramat Gan Israel"},{"name":"Sackler Faculty of Medicine Tel Aviv University Tel Aviv Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,7,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)55188-7"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmg005"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)55548-4"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1071-5576(97)00113-5"},{"key":"e_1_2_7_6_1","first-page":"78","volume-title":"Basic and clinical immunology","author":"Oppenheim JJ","year":"1991"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-6060(97)90333-0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/10.1.24"},{"key":"e_1_2_7_9_1","first-page":"297","article-title":"Interleukin\u20102 production by cultured human granulosa cells","volume":"42","author":"Orvieto R","year":"1997","journal-title":"Int J Fertil Womens Med"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13048-014-0106-2"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1053\/S0046-8177(03)00339-3"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.2001.01147.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsgi.2004.05.001"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/12.12.2629"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1999.tb00103.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.8755-8920.2000.440501.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/gye.15.1.29.33"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Faji.12416","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.12416","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T13:23:05Z","timestamp":1696512185000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.12416"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,28]]},"references-count":16,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["10.1111\/aji.12416"],"URL":"https:\/\/doi.org\/10.1111\/aji.12416","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,28]]}}}