{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T07:15:55Z","timestamp":1695798955539},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,10,13]],"date-time":"2015-10-13T00:00:00Z","timestamp":1444694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>Identification of the types of cells that produce <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 in the genital tract can clarify the roles that these cytokines play in responses to pathogens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>We isolated and stimulated cells from cervical tissue to identify and characterize cytokine\u2010producing cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Upon stimulation of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> endocervical cells, 1.6, 3.4, and 1.5% were induced to produce <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017, and both cytokines, respectively. Stimulation of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> ectocervical cells resulted in 3.3% <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022<jats:sup>+<\/jats:sup>, 5.5% <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017<jats:sup>+<\/jats:sup> and 2.6% <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>17<jats:sup>+<\/jats:sup> cells. <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3<jats:sup>\u2212<\/jats:sup> cells had relatively high endogenous levels of cytokine expression that did not increase upon stimulation. Innate lymphoid cells (<jats:styled-content style=\"fixed-case\">ILC<\/jats:styled-content>s) made up 5.7\u20138% of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45<jats:sup>+<\/jats:sup> cervical cells and stimulation caused increases in <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These studies show that the majority of the <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45<jats:sup>+<\/jats:sup> leukocytes that can be induced to produce <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017 in cervix are <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup>, but <jats:styled-content style=\"fixed-case\">ILC<\/jats:styled-content>s are also present and can make both cytokines.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12435","type":"journal-article","created":{"date-parts":[[2015,10,13]],"date-time":"2015-10-13T14:56:51Z","timestamp":1444748211000},"page":"42-50","source":"Crossref","is-referenced-by-count":2,"title":["Characterization of <scp>IL<\/scp>\u201022 and <scp>IL<\/scp>\u201017 Expressing Leukocytes in the Cervix"],"prefix":"10.1111","volume":"75","author":[{"given":"Hadijat M.","family":"Makinde","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology Rush University Medical Center Chicago IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nell","family":"Lurain","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Rush University Medical Center Chicago IL 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