{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T09:20:46Z","timestamp":1780392046354,"version":"3.54.1"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,1,18]],"date-time":"2016-01-18T00:00:00Z","timestamp":1453075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Excellent Youth and Middle Age Scientists Fund of Shandong Province","award":["BS2012SW013"],"award-info":[{"award-number":["BS2012SW013"]}]},{"name":"Science and Technology Research Found from Population and Family Planning Commission of Shandong Province","award":["201103"],"award-info":[{"award-number":["201103"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2016,5]]},"abstract":"Problem<\/jats:title>To study the effect of indoleamine 2,3\u2010dioxygenase (IDO<\/jats:styled-content>) expressed in HTR<\/jats:styled-content>\u20108\/SV<\/jats:styled-content>neo cells on NKG<\/jats:styled-content>2D and NK<\/jats:styled-content>p46 expression and cytotoxicity of decidual NK<\/jats:styled-content> (dNK<\/jats:styled-content>) and peripheral NK<\/jats:styled-content> (pNK<\/jats:styled-content>) cells.<\/jats:p><\/jats:sec>Method of study<\/jats:title>CD<\/jats:styled-content>56+<\/jats:sup> dNK<\/jats:styled-content> and pNK<\/jats:styled-content> cells purified were cultured with HTR<\/jats:styled-content>\u20108\/SV<\/jats:styled-content>neo cell conditioned medium (CM<\/jats:styled-content>), 1\u2010MT<\/jats:styled-content>+HTR<\/jats:styled-content>\u20108\/SV<\/jats:styled-content>neo cell CM<\/jats:styled-content>, and complete RPMI<\/jats:styled-content> 1640 medium (negative control) in vitro<\/jats:italic>. The mRNA<\/jats:styled-content> and protein expression of NKG<\/jats:styled-content>2D and NK<\/jats:styled-content>p46 in NK<\/jats:styled-content> cells were then assessed by qRT<\/jats:styled-content>\u2010PCR<\/jats:styled-content> and flow cytometry, respectively. Their cytotoxicity was evaluated with LDH<\/jats:styled-content> assays, and TNF<\/jats:styled-content>\u2010\u03b1 secretion was analyzed by ELISA.<\/jats:p><\/jats:sec>Results<\/jats:title>For dNK<\/jats:styled-content> cells, the mRNA<\/jats:styled-content> and protein expression of NK<\/jats:styled-content>p46 as well as NKG<\/jats:styled-content>2D did not differ significantly among the three groups (P<\/jats:italic> > 0.05), whereas for pNK<\/jats:styled-content> cells, the expression level was significantly decreased in HTR<\/jats:styled-content>\u20108\/SV<\/jats:styled-content>neo cell CM<\/jats:styled-content> group than the other two groups (P<\/jats:italic> < 0.01). Peripheral NK<\/jats:styled-content> cells cultured with HTR<\/jats:styled-content>\u20108\/SV<\/jats:styled-content>neo cell CM<\/jats:styled-content> showed reduced cytotoxicity and TNF<\/jats:styled-content>\u2010\u03b1 secretion than the other two groups (P<\/jats:italic> < 0.01), although there were no significant differences among three groups for dNK<\/jats:styled-content> cells (P<\/jats:italic> > 0.05).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>IDO<\/jats:styled-content> expressed by HTR<\/jats:styled-content>\u20108\/SV<\/jats:styled-content>neo cells can down\u2010regulate NK<\/jats:styled-content>p46 and NKG<\/jats:styled-content>2D expression and reduce cytotoxicity in pNK<\/jats:styled-content> cells, and may contribute to keep dNK<\/jats:styled-content> cytotoxicity at a low level, suggesting an important role for IDO<\/jats:styled-content> in the maintenance of normal pregnancy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12481","type":"journal-article","created":{"date-parts":[[2016,1,19]],"date-time":"2016-01-19T05:09:27Z","timestamp":1453180167000},"page":"519-528","source":"Crossref","is-referenced-by-count":20,"title":["Effect of Indoleamine 2,3\u2010Dioxygenase Expressed in HTR<\/scp>\u20108\/SV<\/scp>neo Cells on Decidual NK<\/scp> Cell Cytotoxicity"],"prefix":"10.1111","volume":"75","author":[{"given":"Yanli","family":"Ban","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Qilu Hospital of Shandong University Ji'nan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Qilu Hospital of Shandong University Ji'nan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of 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