{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:57:25Z","timestamp":1790816245661,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,2,10]],"date-time":"2017-02-10T00:00:00Z","timestamp":1486684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["FDN 40005179"],"award-info":[{"award-number":["FDN 40005179"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP130550"],"award-info":[{"award-number":["MOP130550"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003579","name":"University of Toronto","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003579","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:sec>\n                    <jats:title>Problem<\/jats:title>\n                    <jats:p>The extravillous trophoblasts (EVT) express HLA\u2010C and HLA\u2010G, but HLA\u2010E and HLA\u2010F are the subject of conflicting reports. In this study, we define the HLA expression profile during active EVT placental implantation, pregnancy development, and parturition.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method of study<\/jats:title>\n                    <jats:p>Immunohistochemistry, q\u2010PCR, and Western blot were used to investigate HLA\u2010C, HLA\u2010E, and HLA\u2010F placental expression across gestation from the early first trimester, late first trimester, second trimester (n=10 in each), preterm gestation (n=6) to elective term cesarean section and term vaginal deliveries (n=12, 38\u201041\u00a0weeks). EVT explants and Swan71 cells were used to assess HLA\u2010C and HLA\u2010F during active EVT migration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>HLA\u2010G, HLA\u2010C, and HLA\u2010F were expressed by 1st\u2010trimester EVT and became intracellular and weaker as gestation progressed. HLA\u2010E was only expressed in 1st\u2010trimester placenta. HLA\u2010F and HLA\u2010C mRNA and protein expression levels showed a significant increase in the fetal villous mesenchyme across gestation. HLA\u2010C levels increased with labor. We detected a 100\u2010kDa HLA\u2010F band in early pregnancy suggesting dimer formation on the EVT surface. These results were confirmed in EVT outgrowths and Swan71 trophoblast which showed that HLA\u2010F and HLA\u2010G are increased on the cell surface of migrating EVT, while HLA\u2010C was internalized.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Expression of HLA\u2010F and HLA\u2010G on the cell surface of actively migrating EVT supports their specific role in early EVT invasion and interactions with uterine natural killer cells. HLA\u2010C's limited expression to the proliferative EVT suggests a protective role in the earliest events of implantation but not in active EVT invasion. We also show for the first time that HLA\u2010C may be involved in parturition.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.12643","type":"journal-article","created":{"date-parts":[[2017,2,10]],"date-time":"2017-02-10T00:06:54Z","timestamp":1486685214000},"source":"Crossref","is-referenced-by-count":134,"title":["Definitive class I human leukocyte antigen expression in gestational placentation:\n                    <scp>HLA<\/scp>\n                    \u2010F,\n                    <scp>HLA<\/scp>\n                    \u2010E,\n                    <scp>HLA<\/scp>\n                    \u2010C, and\n                    <scp>HLA<\/scp>\n                    \u2010G in extravillous trophoblast invasion on placentation, pregnancy, and parturition"],"prefix":"10.1111","volume":"77","author":[{"given":"Rinat","family":"Hackmon","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology University of Toronto, Mount Sinai Hospital  Toronto ON Canada"},{"name":"Clinical Research Division Fred Hutchinson Cancer Research Center  Seattle WA USA"},{"name":"Division of MFM Obstetrics and Gynecology OHSU  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lakmini","family":"Pinnaduwage","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Toronto  Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Lunenfeld Tanenbaum Research Institute Mount Sinai Hospital  Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J.","family":"Lye","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University of Toronto, Mount Sinai Hospital  Toronto ON Canada"},{"name":"Department of Physiology University of Toronto  Toronto ON Canada"},{"name":"Lunenfeld Tanenbaum Research Institute Mount Sinai Hospital  Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel E.","family":"Geraghty","sequence":"additional","affiliation":[{"name":"Clinical Research Division Fred Hutchinson Cancer Research Center  Seattle WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4146-099X","authenticated-orcid":false,"given":"Caroline E.","family":"Dunk","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University of Toronto, Mount Sinai Hospital  Toronto ON Canada"},{"name":"Lunenfeld Tanenbaum Research Institute Mount Sinai Hospital  Toronto ON 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