{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:00:26Z","timestamp":1791223226895,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,2,27]],"date-time":"2017-02-27T00:00:00Z","timestamp":1488153600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004751","name":"Department of S and T for Social Development","doi-asserted-by":"publisher","award":["2015CB943300"],"award-info":[{"award-number":["2015CB943300"]}],"id":[{"id":"10.13039\/501100004751","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471548"],"award-info":[{"award-number":["81471548"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81200425"],"award-info":[{"award-number":["81200425"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:sec><jats:label\/><jats:p>Endometriosis is a chronic inflammatory disease characterized by the elevation of proinflammatory cytokines, such as <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106, in the peritoneal fluid. However, the precise mechanism of the highly elevated <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 levels in ectopic milieu remains unclear. The aim of this study was to investigate whether the cross talk between endometrial stromal cells (<jats:styled-content style=\"fixed-case\">ESC<\/jats:styled-content>s) and macrophages contributes to the elevated <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 production. Samples of endometrium and ectopic tissues were obtained from patients with or without endometriosis. The peripheral blood samples were collected from healthy volunteers. Enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>) was for <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 levels in peritoneal fluid and cell culture supernatant. In\u2010Cell Western assay was used for protein expression of <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>17 and phosphorylation levels of <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> JNK<\/jats:styled-content>, and P38. Immunohistochemistry was performed on normal, eutopic endometrium and ectopic tissues to analyze <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>17 expression. Flow cytometry was applied to detect the expression of <jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>4, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106, and the phosphorylation levels of <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB. Patients with endometriosis have higher levels of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 in peritoneal fluid compared to the control. The co\u2010culture of <jats:styled-content style=\"fixed-case\">ESC<\/jats:styled-content>s and macrophages produce more <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 than cultured alone, respectively. The eutopic endometrium had significantly higher expression of <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>17 compared to normal endometrium, and the ectopic tissues had the highest expression. <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 induced <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>17 secretion in <jats:styled-content style=\"fixed-case\">ESC<\/jats:styled-content>s via activating <jats:styled-content style=\"fixed-case\">JNK<\/jats:styled-content> signaling pathway, <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>17 upregulated the expression of its receptor <jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>4 on macrophages. Furthermore, <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>17\u2010<jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>4 axis subsequently led to excessive <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 production in macrophages by activating <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB. These findings suggest that the cross talk between <jats:styled-content style=\"fixed-case\">ESC<\/jats:styled-content>s and macrophages promotes the expression of <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>17 in <jats:styled-content style=\"fixed-case\">ESC<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>4 on macrophages, which contributes to the high levels of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 in ectopic milieu.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12644","type":"journal-article","created":{"date-parts":[[2017,2,27]],"date-time":"2017-02-27T10:33:32Z","timestamp":1488191612000},"source":"Crossref","is-referenced-by-count":18,"title":["The <scp>CCL<\/scp>17\u2010<scp>CCR<\/scp>4 axis between endometrial stromal cells and macrophages contributes to the high levels of <scp>IL<\/scp>\u20106 in ectopic milieu"],"prefix":"10.1111","volume":"78","author":[{"given":"Wen\u2010Jie","family":"Zhou","sequence":"first","affiliation":[{"name":"Laboratory for Reproductive Immunology Key Laboratory of Reproduction Regulation of NPFPC SIPPR Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Hospital and Institute of Obstetrics and Gynecology IRD Fudan University Shanghai Medical College  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010Xin","family":"Hou","sequence":"additional","affiliation":[{"name":"Laboratory for Reproductive Immunology Key Laboratory of Reproduction Regulation of NPFPC SIPPR Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Hospital and Institute of Obstetrics and Gynecology IRD Fudan University Shanghai Medical College  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Qiu","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory for Reproductive Immunology Key Laboratory of Reproduction Regulation of NPFPC SIPPR Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Hospital and Institute of Obstetrics and Gynecology IRD Fudan University Shanghai Medical College  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da\u2010Jin","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory for Reproductive Immunology Key Laboratory of Reproduction Regulation of NPFPC SIPPR Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Hospital and Institute of Obstetrics and Gynecology IRD Fudan University Shanghai Medical College  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2014.12.019"},{"key":"e_1_2_6_3_1","first-page":"199","article-title":"Medical management of endometriosis: emerging evidence linking inflammation to disease pathophysiology","volume":"65","author":"Bruner\u2010Tran KL","year":"2013","journal-title":"Minerva Ginecol"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)17403-5"},{"key":"e_1_2_6_5_1","first-page":"1042","article-title":"Role of cytokines in pathogenesis of endometriosis","volume":"6","author":"Barcz E","year":"2000","journal-title":"Med Sci Monit"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(97)81439-2"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a016295"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2008.11.004"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/149185"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2005.11.060"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.81.3.1118"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0897.2003.00042.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/den393"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/17.4.1039"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.03.057"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0028-1082387"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/den125"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.20113"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)58262-4"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1159\/000052857"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/795976"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2007.02.057"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1159\/000067717"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/17.1.69"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)55216-9"},{"key":"e_1_2_6_26_1","first-page":"237","article-title":"Immunology of endometriosis","volume":"57","author":"Ulukus M","year":"2005","journal-title":"Minerva Ginecol"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/4.5.741"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/der410"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(97)00191-X"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0001-6349.2004.00229.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2008.08.007"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2008.09.002"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3153"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2865"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/del516"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2013.02.038"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2013.03.003"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-2767"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Faji.12644","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.12644","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T20:20:12Z","timestamp":1694550012000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.12644"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,27]]},"references-count":37,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["10.1111\/aji.12644"],"URL":"https:\/\/doi.org\/10.1111\/aji.12644","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,27]]},"article-number":"e12644"}}