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The second step includes assembly of the sensor molecule and adaptor protein ASC<\/jats:styled-content> (an apoptosis\u2010associated speck\u2010like protein containing a CARD), which results in ASC<\/jats:styled-content> speck formation and the recruitment of caspase (CASP<\/jats:styled-content>)\u20101. Herein, we investigated whether there is inflammasome assembly in the chorioamniotic membranes and choriodecidual leukocytes from women who underwent spontaneous labor at term.<\/jats:p><\/jats:sec>Method of Study<\/jats:title>Using in situ proximity ligation assays, ASC<\/jats:styled-content>\/CASP<\/jats:styled-content>\u20101 complexes were determined in the chorioamniotic membranes from women who delivered at term without labor or underwent spontaneous labor at term with or without acute histologic chorioamnionitis (n=10\u201011 each). Also, ASC<\/jats:styled-content> speck formation was determined by flow cytometry in the choriodecidual leukocytes isolated from women who delivered at term with or without spontaneous labor (n=9\u201012 each).<\/jats:p><\/jats:sec>Results<\/jats:title>(i) ASC<\/jats:styled-content>\/CASP<\/jats:styled-content>\u20101 complexes were detected in the chorioamniotic membranes; (ii) ASC<\/jats:styled-content>\/CASP<\/jats:styled-content>\u20101 complexes were greater in the chorioamniotic membranes from women who underwent spontaneous labor at term than in those without labor; (iii) ASC<\/jats:styled-content>\/CASP<\/jats:styled-content>\u20101 complexes were even more abundant in the chorioamniotic membranes from women who underwent spontaneous labor at term with acute histologic chorioamnionitis than in those without this placental lesion; (iv) ASC<\/jats:styled-content> speck formation was detected in the choriodecidual leukocytes; and (v) ASC<\/jats:styled-content> speck formation was greater in the choriodecidual leukocytes isolated from women who underwent spontaneous labor at term than in those without labor.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>There is inflammasome assembly in the chorioamniotic membranes and choriodecidual leukocytes during spontaneous labor at term.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12648","type":"journal-article","created":{"date-parts":[[2017,2,24]],"date-time":"2017-02-24T07:09:42Z","timestamp":1487920182000},"source":"Crossref","is-referenced-by-count":39,"title":["Inflammasome assembly in the chorioamniotic membranes during spontaneous labor at term"],"prefix":"10.1111","volume":"77","author":[{"given":"Nardhy","family":"Gomez\u2010Lopez","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Wayne State University School of Medicine Detroit MI USA"},{"name":"Perinatology Research Branch Program for Perinatal Research and Obstetrics Division of Intramural Research Eunice Kennedy Shriver<\/i> 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