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The placenta as an immune regulatory organ expresses <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>s, leading to the secretion of cytokines. Both parasites are recognized by <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>\u20102, <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>\u20104, and <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>\u20109. Here, we studied if the parasites induce differences in <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content> protein expression, cytokine profiles, and whether receptor inhibition is related to parasite infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Placental tissue explants were infected <jats:italic>ex vivo<\/jats:italic> with each parasite, <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>s protein expression, cytokine profile and parasite infection were determined by Western blotting, <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Trypanosoma cruzi<\/jats:italic> and <jats:italic>Toxoplasma gondii<\/jats:italic> infection is related to <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>\u20102 and <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>\u20104\/<jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>\u20109, respectively. <jats:italic>Trypanosoma cruzi<\/jats:italic> elicits an increase in <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 cytokine secretion whereas <jats:italic>T.\u00a0gondii<\/jats:italic> only increases the secretion of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The susceptibility of the placenta to each parasite is mediated partially by the innate immune 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