{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T04:45:17Z","timestamp":1767847517258,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,3,24]],"date-time":"2017-03-24T00:00:00Z","timestamp":1490313600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1047025"],"award-info":[{"award-number":["1047025"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1058786"],"award-info":[{"award-number":["1058786"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2017,7]]},"abstract":"Problem<\/jats:title>TNF<\/jats:styled-content>\u2010\u03b1 plays a central role in the processes of human labour and delivery. This study sought to determine the role of the adaptor proteins TNFR<\/jats:styled-content>1\u2010associated death domain protein (TRADD<\/jats:styled-content>), TNF<\/jats:styled-content> receptor\u2010associated factor 2 (TRAF<\/jats:styled-content>2), receptor interacting protein 1 (RIP<\/jats:styled-content>1) and transforming growth factor beta\u2010activated kinase 1 (TAK<\/jats:styled-content>1) in TNF<\/jats:styled-content>\u2010\u03b1\u2010induced formation of pro\u2010labour mediators.<\/jats:p><\/jats:sec>Method of study<\/jats:title>Human primary myometrial cells were transfected with siRNA<\/jats:styled-content> against TRADD<\/jats:styled-content> (siTRADD<\/jats:styled-content>), TRAF<\/jats:styled-content>2 (siTRAF<\/jats:styled-content>2), RIP<\/jats:styled-content>1 (siRIP<\/jats:styled-content>1) or TAK<\/jats:styled-content>1 (siTAK<\/jats:styled-content>1), treated with TNF<\/jats:styled-content>\u2010\u03b1, and assayed for pro\u2010inflammatory mediators expression.<\/jats:p><\/jats:sec>Results<\/jats:title>siTRADD<\/jats:styled-content>, siTRAF<\/jats:styled-content>2, siRIP<\/jats:styled-content>1 and siTAK<\/jats:styled-content>1 significantly decreased TNF<\/jats:styled-content>\u2010\u03b1\u2010induced IL<\/jats:styled-content>\u20101\u03b1, IL<\/jats:styled-content>\u20101\u03b2, IL<\/jats:styled-content>\u20106, IL<\/jats:styled-content>\u20108, MCP<\/jats:styled-content>\u20101 mRNA<\/jats:styled-content> expression and release of IL<\/jats:styled-content>\u20106, IL<\/jats:styled-content>\u20108 and MCP<\/jats:styled-content>\u20101; and cyclooxygenase (COX<\/jats:styled-content>)\u20102 expression and release of prostaglandin PGF<\/jats:styled-content>2\u03b1<\/jats:sub>. There was a significant attenuation of TNF<\/jats:styled-content>\u2010\u03b1\u2010induced expression of adhesion molecules ICAM<\/jats:styled-content>\u20101 and VCAM<\/jats:styled-content>\u20101 mRNA<\/jats:styled-content> with siTRADD<\/jats:styled-content>, siTRAF<\/jats:styled-content>2 or siRIP<\/jats:styled-content>1. siTRADD<\/jats:styled-content> and siRIP<\/jats:styled-content>1 significantly attenuated TNF<\/jats:styled-content>\u2010\u03b1\u2010induced MMP<\/jats:styled-content>\u20109 mRNA<\/jats:styled-content> expression and release and nuclear factor \u03baB (NF<\/jats:styled-content>\u2010\u03baB) transcriptional activity. There was a significant increase in TNF<\/jats:styled-content>\u2010\u03b1\u2010induced sVCAM<\/jats:styled-content>\u20101 release, MMP<\/jats:styled-content>\u20109 mRNA<\/jats:styled-content> expression and NF<\/jats:styled-content>\u2010\u03baB activity with siTAK1.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>TRADD<\/jats:styled-content>, TRAF<\/jats:styled-content>2, RIP<\/jats:styled-content>1 and TAK<\/jats:styled-content>1 are involved in TNF<\/jats:styled-content>\u2010\u03b1 signalling in human myometrium. Further studies are required to determine whether inhibition of these proteins can prevent preterm birth.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12664","type":"journal-article","created":{"date-parts":[[2017,3,24]],"date-time":"2017-03-24T11:57:43Z","timestamp":1490356663000},"source":"Crossref","is-referenced-by-count":18,"title":["TRADD<\/scp>, TRAF<\/scp>2, RIP<\/scp>1 and TAK<\/scp>1 are required for TNF<\/scp>\u2010\u03b1\u2010induced pro\u2010labour mediators in human primary myometrial cells"],"prefix":"10.1111","volume":"78","author":[{"given":"Ratana","family":"Lim","sequence":"first","affiliation":[{"name":"Obstetrics Nutrition and Endocrinology Group Department of Obstetrics and Gynaecology University of Melbourne Heidelberg Victoria Australia"},{"name":"Mercy Perinatal Research Centre Mercy Hospital for Women Heidelberg Victoria 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