{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T15:44:59Z","timestamp":1762875899829,"version":"3.41.2"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,6,7]],"date-time":"2017-06-07T00:00:00Z","timestamp":1496793600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Grant-in-Aid for Scientific Research","award":["26293358, 26293103"],"award-info":[{"award-number":["26293358, 26293103"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">APOBEC<\/jats:styled-content>3G (A3G) is a cytidine deaminase that exhibits antiviral activity by introducing C\u2010to\u2010T hypermutation in viral <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. We recently observed the distinct presence of C\u2010to\u2010T hypermutation of human papillomavirus <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> in uterine cervical intraepithelial neoplasia (<jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>), suggesting the possible involvement of A3G in the mutation\u2010inducing process. Consequently, we investigated the association of A3G expression with <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content> progression in this study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Patients who had undergone cervical conization due to <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>1 (n=11), <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>2 (n=9), <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>3 (n=12), and micro\u2010invasive squamous cell carcinoma (n=2) were included. The expression profiles of A3G and p16 proteins in cervical lesions and A3G\u2010positive immune cells around the lesions were examined by immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunoreactive A3G protein was detected in the <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content> and squamous cell carcinoma lesions. Its expression intensity and positive areas were increased and spread in accordance with the progression of <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>, respectively. The co\u2010expression of p16 was observed on the A3G\u2010positive atypical cells. The numbers of A3G\u2010positive immune cells in <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>3 lesions were significantly higher than those of <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>1\u20102 lesions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings indicate that A3G is associated with <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>, suggesting its important roles in human papillomavirus\u2010induced pathophysiological processes such as <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content> progression and viral elimination.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12703","type":"journal-article","created":{"date-parts":[[2017,6,7]],"date-time":"2017-06-07T10:57:02Z","timestamp":1496833022000},"source":"Crossref","is-referenced-by-count":10,"title":["<scp>APOBEC<\/scp>3G is increasingly expressed on the human uterine cervical intraepithelial neoplasia along with disease progression"],"prefix":"10.1111","volume":"78","author":[{"given":"Takashi","family":"Iizuka","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics Graduate School of Medical Science Kanazawa University  Kanazawa Japan"},{"name":"Department of Obstetrics and Gynecology Graduate School of Medical Science Kanazawa University  Kanazawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kousho","family":"Wakae","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics Graduate School of Medical Science Kanazawa University  Kanazawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuhiro","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Graduate School of Medical Science Kanazawa University  Kanazawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Kitamura","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics Graduate School of Medical Science Kanazawa University  Kanazawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masanori","family":"Ono","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Graduate School of Medical Science Kanazawa University  Kanazawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3098-9406","authenticated-orcid":false,"given":"Hiroshi","family":"Fujiwara","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Graduate School of Medical Science Kanazawa University  Kanazawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masamichi","family":"Muramatsu","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics Graduate School of Medical Science Kanazawa University  Kanazawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,6,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21262"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1002\/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F","article-title":"Human papillomavirus is a necessary cause of invasive cervical cancer worldwide","volume":"89","author":"Walboomers JM","year":"1999","journal-title":"J Pathol"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1489"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02587-12"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.03.012"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02383-14"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.08.017"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.4103\/2277-9175.124626"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2701"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2702"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2015.02.007"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-15-0344"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24215"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jog.13049"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003361"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1827.1999.00875.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2010.11.003"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_8_20_1","first-page":"4620","article-title":"E7 protein of human papilloma virus\u201016 induces degradation of retinoblastoma protein through the ubiquitin\u2010proteasome pathway","volume":"56","author":"Boyer SN","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2537532"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-15-2910"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2016.335"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03091-13"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051512"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4690-6-38"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00206-06"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20091933"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.7.3159"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Faji.12703","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.12703","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T13:00:06Z","timestamp":1696338006000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.12703"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,7]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,10]]}},"alternative-id":["10.1111\/aji.12703"],"URL":"https:\/\/doi.org\/10.1111\/aji.12703","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"type":"print","value":"1046-7408"},{"type":"electronic","value":"1600-0897"}],"subject":[],"published":{"date-parts":[[2017,6,7]]},"article-number":"e12703"}}