{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,24]],"date-time":"2025-08-24T01:20:40Z","timestamp":1755998440109,"version":"3.41.2"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,5,15]],"date-time":"2017-05-15T00:00:00Z","timestamp":1494806400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471513, 81302260"],"award-info":[{"award-number":["81471513, 81302260"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003347","name":"Fudan University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"publisher","award":["17ZR1403200"],"award-info":[{"award-number":["17ZR1403200"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20140170"],"award-info":[{"award-number":["BK20140170"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2017,10]]},"abstract":"Problem<\/jats:title>To explore whether cervical carcinoma cell\u2010derived interleukin\u201027 (IL<\/jats:styled-content>\u201027) modulates the angiogenesis of vascular endothelial cells.<\/jats:p><\/jats:sec>Method of study<\/jats:title>The expression of IL<\/jats:styled-content>\u201027 in cervical cancer tissues and cervical cell lines was analyzed by immunohistochemistry, ELISA<\/jats:styled-content> and flow cytometry. Then, the effects of IL<\/jats:styled-content>\u201027 on the proliferation and apoptosis\u2010related molecules and angiogenesis in vitro of human umbilical vein endothelial cells (HUVEC<\/jats:styled-content>s) were investigated. Finally, in vivo experiment was performed to further confirm the effects of IL\u201027.<\/jats:p><\/jats:sec>Results<\/jats:title>Compared with cervicitis, the cervical cancer tissues highly expressed IL<\/jats:styled-content>\u201027. Both HeLa and CaSki cells secreted IL<\/jats:styled-content>\u201027, and HUVEC<\/jats:styled-content>s expressed low levels of IL<\/jats:styled-content>\u201027 receptors (IL<\/jats:styled-content>\u201027R). However, the co\u2010culture of cervical cell lines and HUVEC<\/jats:styled-content>s led to a significant elevation of IL<\/jats:styled-content>\u201027R on HUVEC<\/jats:styled-content>s. Co\u2010culturing with IL<\/jats:styled-content>\u201027\u2010overexpressed HeLa cells downregulated Ki\u201067 and Bcl\u20102 and upregulated Fas expression in HUVEC<\/jats:styled-content>s. In addition, overexpression of IL<\/jats:styled-content>\u201027 in HeLa cells and CasKi cells secreted less IL<\/jats:styled-content>\u20108 and could further restrict angiogenesis compared with control cells in vitro. In the subcutaneous tumorous model of C57\/BL<\/jats:styled-content>6 mouse, there were decreased vessel density and tumor volume when inoculation with IL<\/jats:styled-content>\u201027\u2010overexpressed TC<\/jats:styled-content>\u20101 cells.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>This study indicates that IL<\/jats:styled-content>\u201027 secreted by cervical carcinoma cells restricts the angiogenesis in a paracrine manner in the pathogenesis of cervical cancer.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12706","type":"journal-article","created":{"date-parts":[[2017,5,16]],"date-time":"2017-05-16T04:06:42Z","timestamp":1494907602000},"source":"Crossref","is-referenced-by-count":8,"title":["The cross talk between cervical carcinoma cells and vascular endothelial cells mediated by IL<\/scp>\u201027 restrains angiogenesis"],"prefix":"10.1111","volume":"78","author":[{"given":"Bing","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology The Affiliated Hospital of Jiangnan University Wuxi Jiangsu Province China"},{"name":"Laboratory for Reproductive Immunology Hospital of Obstetrics and Gynecology Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Xie","sequence":"additional","affiliation":[{"name":"Laboratory for Reproductive Immunology Hospital of Obstetrics and Gynecology Fudan University Shanghai China"},{"name":"Medical Center of Diagnosis and Treatment for Cervical Diseases Hospital of Obstetrics and Gynecology Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun\u2010Lin","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Affiliated Hospital of Jiangnan University Wuxi Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun\u2010Jie","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Affiliated Hospital of Jiangnan University Wuxi Jiangsu Province China"},{"name":"Laboratory for Reproductive Immunology Hospital of Obstetrics and Gynecology Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiao","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Affiliated Hospital of Jiangnan University Wuxi Jiangsu Province China"},{"name":"Laboratory for Reproductive Immunology Hospital of Obstetrics and Gynecology Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Affiliated Hospital of Jiangnan University Wuxi Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi\u2010Zhong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Affiliated Hospital of Jiangnan University Wuxi Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Pu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Affiliated Hospital of Jiangnan University Wuxi Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Bo","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Affiliated Hospital of Jiangnan University Wuxi Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Wei","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Affiliated Hospital of Jiangnan University Wuxi Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da\u2010Jin","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory for Reproductive Immunology Hospital of Obstetrics and Gynecology Fudan University Shanghai China"},{"name":"Key Laboratory of Reproduction Regulation of NPFPC SIPPR IRD Fudan University Shanghai China"},{"name":"Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin\u2010Jin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Affiliated Hospital of Jiangnan University Wuxi Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9276-0722","authenticated-orcid":false,"given":"Ming\u2010Qing","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory for Reproductive Immunology Hospital of Obstetrics and Gynecology Fudan University Shanghai China"},{"name":"Key Laboratory of Reproduction Regulation of NPFPC SIPPR IRD Fudan University Shanghai China"},{"name":"Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,5,15]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_7_2_1","DOI":"10.1002\/ijc.25516"},{"doi-asserted-by":"publisher","key":"e_1_2_7_3_1","DOI":"10.3322\/caac.21338"},{"doi-asserted-by":"publisher","key":"e_1_2_7_4_1","DOI":"10.1634\/theoncologist.2013-0123"},{"doi-asserted-by":"publisher","key":"e_1_2_7_5_1","DOI":"10.1073\/pnas.0806582105"},{"doi-asserted-by":"publisher","key":"e_1_2_7_6_1","DOI":"10.1006\/bbrc.1998.8576"},{"doi-asserted-by":"publisher","key":"e_1_2_7_7_1","DOI":"10.1038\/35038103"},{"doi-asserted-by":"publisher","key":"e_1_2_7_8_1","DOI":"10.1073\/pnas.0702388104"},{"doi-asserted-by":"publisher","key":"e_1_2_7_9_1","DOI":"10.1158\/0008-5472.CAN-08-4311"},{"doi-asserted-by":"publisher","key":"e_1_2_7_10_1","DOI":"10.1016\/j.imbio.2013.06.001"},{"doi-asserted-by":"publisher","key":"e_1_2_7_11_1","DOI":"10.18632\/oncotarget.1425"},{"doi-asserted-by":"publisher","key":"e_1_2_7_12_1","DOI":"10.1002\/ijc.24107"},{"doi-asserted-by":"publisher","key":"e_1_2_7_13_1","DOI":"10.1038\/ncomms7072"},{"doi-asserted-by":"publisher","key":"e_1_2_7_14_1","DOI":"10.1186\/1756-9966-32-97"},{"key":"e_1_2_7_15_1","first-page":"21","article-title":"Treatment of established tumors with a novel vaccine that enhances major histocompatibility class II presentation of tumor antigen","volume":"56","author":"Lin KY","year":"1996","journal-title":"Cancer Res"},{"doi-asserted-by":"publisher","key":"e_1_2_7_16_1","DOI":"10.1242\/jcs.037507"},{"doi-asserted-by":"publisher","key":"e_1_2_7_17_1","DOI":"10.1097\/MOH.0000000000000239"},{"doi-asserted-by":"publisher","key":"e_1_2_7_18_1","DOI":"10.3322\/caac.21262"},{"doi-asserted-by":"publisher","key":"e_1_2_7_19_1","DOI":"10.1016\/S0092-8674(00)80108-7"},{"doi-asserted-by":"publisher","key":"e_1_2_7_20_1","DOI":"10.1038\/427787a"},{"doi-asserted-by":"publisher","key":"e_1_2_7_21_1","DOI":"10.1002\/bies.10351"},{"doi-asserted-by":"publisher","key":"e_1_2_7_22_1","DOI":"10.1038\/ni1540"},{"doi-asserted-by":"publisher","key":"e_1_2_7_23_1","DOI":"10.1038\/ni1537"},{"doi-asserted-by":"publisher","key":"e_1_2_7_24_1","DOI":"10.1038\/ni.1996"},{"doi-asserted-by":"publisher","key":"e_1_2_7_25_1","DOI":"10.1084\/jem.20131548"},{"doi-asserted-by":"publisher","key":"e_1_2_7_26_1","DOI":"10.4049\/jimmunol.180.5.2752"},{"doi-asserted-by":"publisher","key":"e_1_2_7_27_1","DOI":"10.4049\/jimmunol.173.9.5626"},{"doi-asserted-by":"publisher","key":"e_1_2_7_28_1","DOI":"10.4049\/jimmunol.175.3.1686"},{"doi-asserted-by":"publisher","key":"e_1_2_7_29_1","DOI":"10.1002\/eji.201040804"},{"doi-asserted-by":"publisher","key":"e_1_2_7_30_1","DOI":"10.1038\/ni1541"},{"doi-asserted-by":"publisher","key":"e_1_2_7_31_1","DOI":"10.1038\/ni1540"},{"doi-asserted-by":"publisher","key":"e_1_2_7_32_1","DOI":"10.1189\/jlb.1209788"},{"doi-asserted-by":"publisher","key":"e_1_2_7_33_1","DOI":"10.4049\/jimmunol.1103382"},{"doi-asserted-by":"publisher","key":"e_1_2_7_34_1","DOI":"10.1016\/j.immuni.2012.03.024"},{"doi-asserted-by":"publisher","key":"e_1_2_7_35_1","DOI":"10.1038\/ncomms7072"},{"doi-asserted-by":"publisher","key":"e_1_2_7_36_1","DOI":"10.4049\/jimmunol.176.12.7317"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Faji.12706","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.12706","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T13:00:22Z","timestamp":1696338022000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.12706"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,15]]},"references-count":35,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,10]]}},"alternative-id":["10.1111\/aji.12706"],"URL":"https:\/\/doi.org\/10.1111\/aji.12706","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"type":"print","value":"1046-7408"},{"type":"electronic","value":"1600-0897"}],"subject":[],"published":{"date-parts":[[2017,5,15]]},"article-number":"e12706"}}