{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T00:30:04Z","timestamp":1784334604324,"version":"3.55.0"},"reference-count":49,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,6,13]],"date-time":"2017-06-13T00:00:00Z","timestamp":1497312000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005851","name":"Shahid Beheshti University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005851","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2017,11]]},"abstract":"Problem<\/jats:title>The use of in vitro maturation (IVM<\/jats:styled-content>) as an alternative approach to the conventional assisted reproductive technique in clinical practice is limited due to low fertilization rate. The expression of Toll\u2010like receptors (TLR<\/jats:styled-content>s) as members of an innate immune system in cumulus cells are thought to affect fertilization. This study was aimed to evaluate the effect of IVM<\/jats:styled-content> on TLR<\/jats:styled-content>4 gene expression and fertilization rate in oocytes derived from IVM<\/jats:styled-content> in comparison with in vivo matured one.<\/jats:p><\/jats:sec>Method of study<\/jats:title>Cumulus cells are collected from oocytes derived IVM<\/jats:styled-content> and in vivo. The expression level of Tlr4 in cumulus cells of both groups was assessed by quantitative real\u2010time PCR<\/jats:styled-content>. To examine the protein expression level of TLR<\/jats:styled-content>4, immunocytochemistry and Western blotting techniques were carried out. TLR<\/jats:styled-content>4 receptor functions were also confirmed by blockade assay and TLR<\/jats:styled-content>4 receptor activation with lipopolysaccharide.<\/jats:p><\/jats:sec>Result<\/jats:title>There was a substantial decrease in fertilization and blastulation rate in the IVM<\/jats:styled-content> group in comparison with the in vivo one. The mRNA<\/jats:styled-content> expression and protein levels of TLR<\/jats:styled-content>4 declined in cumulus cells following IVM<\/jats:styled-content>. Anti\u2010TLR<\/jats:styled-content>4 blocking antibody dramatically decreased the rate of fertilization and blastocyst formation compared to the in vivo group. In contrast, the fertilization rate was enhanced significantly in the presence of LPS<\/jats:styled-content> as a TLR<\/jats:styled-content>4 ligand compared to the control group.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Low expression level of Tlr4 following IVM<\/jats:styled-content> and higher fertilization rate through TLR<\/jats:styled-content>4 receptor activation with LPS<\/jats:styled-content> proposed that alteration in TLR<\/jats:styled-content>4 expression and subsequent cytokine section could be a possible cause of low fertilization rate in IVM<\/jats:styled-content>\u2010derived oocytes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12720","type":"journal-article","created":{"date-parts":[[2017,6,13]],"date-time":"2017-06-13T07:26:46Z","timestamp":1497338806000},"source":"Crossref","is-referenced-by-count":17,"title":["Toll\u2010like receptor4 as a modulator of fertilization and subsequent pre\u2010implantation development following in vitro maturation in mice"],"prefix":"10.1111","volume":"78","author":[{"given":"Sara","family":"Hosseini","sequence":"first","affiliation":[{"name":"Department of Reproductive Biology and Anatomical Sciences Faculty of Medicine Shahid Beheshti University of Medical Sciences Tehran Iran"},{"name":"Department of Transgenic Animal Science Stem Cell Technology Research Center Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Dehghani\u2010Mohammadabadi","sequence":"additional","affiliation":[{"name":"Department of Transgenic Animal Science Stem Cell Technology Research Center Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marefat","family":"Ghafarri Novin","sequence":"additional","affiliation":[{"name":"Department of Reproductive Biology and Anatomical Sciences Faculty of Medicine Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mostafa","family":"Haji Molla Hoseini","sequence":"additional","affiliation":[{"name":"Department of Immunology Faculty of Medicine Shahid Beheshti University of Medical Science Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ehsan","family":"Arefian","sequence":"additional","affiliation":[{"name":"Department of Microbiology School of Biology College of Science University of Tehran Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samira","family":"Mohammadi Yeganeh","sequence":"additional","affiliation":[{"name":"Department of Biotechnology School of Advanced Technologies in Medicine Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3768-4325","authenticated-orcid":false,"given":"Mohammad","family":"Salehi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology School of Advanced Technologies in Medicine Shahid Beheshti University of Medical Sciences Tehran Iran"},{"name":"Cellular and Molecular Biology Research Center Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,6,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)60038-7"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)61450-2"},{"key":"e_1_2_6_4_1","first-page":"99","article-title":"Embryological aspects of oocyte in vitro maturation","volume":"2","author":"Farsi MM","year":"2013","journal-title":"Int J Mol Cell Med"},{"key":"e_1_2_6_5_1","first-page":"155","article-title":"Developmental competence of in vivo and in vitro matured oocytes: a review","volume":"6","author":"Blanco MR","year":"2011","journal-title":"BMBR"},{"key":"e_1_2_6_6_1","first-page":"77","article-title":"IVM results are comparable and may have advantages over standard IVF","volume":"6","author":"Ellenbogen A","year":"2014","journal-title":"Facts Views Vis Obgyn"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0134696"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deu248"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.04.046"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2012.07.001"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1265680"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.5653\/cerm.2013.40.4.143"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2013.01.141"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2013.01.090"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2007.01.036"},{"key":"e_1_2_6_16_1","volume-title":"Textbook of Assisted Reproductive Technologies","author":"David KG","year":"2012"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-014-0354-5"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.07.002"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009450014064"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-014-0363-4"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-6-58"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0531.2002.00345.x"},{"key":"e_1_2_6_23_1","first-page":"74","article-title":"The Effects of cumulus cells on in vitro maturation of mouse germinal vesicle stage oocytes Iranian","volume":"13","author":"Mahmoudi RSA","year":"2005","journal-title":"J Reprod Med"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00469.2012"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.5653\/cerm.2015.42.3.111"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dep265"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2008.07.1760"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod67.2.575"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.4172\/2165-7491.1000e111"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1391"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-008-9219-0"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.020461"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1863"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00316"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2006-0194"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20001858"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.537936"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-014-0172-9"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2006.09.003"},{"key":"e_1_2_6_40_1","first-page":"1068","volume-title":"Principles & Practice of Assisted Reproductive Technology","author":"Kamini A","year":"2014"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.06.002"},{"key":"e_1_2_6_42_1","first-page":"149","article-title":"In vitro maturation system for individual culture of bovine oocytes using micro\u2010volume multi\u2010well plate","volume":"61","author":"Nagano M","year":"2013","journal-title":"Jpn J Vet Res"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.120135gv"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-010-9490-8"},{"key":"e_1_2_6_45_1","first-page":"19","article-title":"The Effect of Retinoic Acid on in vitro Maturation and Fertilization Rate of Mouse Germinal Vesicle Stage Oocytes","volume":"13","author":"Nasiri E","year":"2011","journal-title":"Cell J"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00249-009-0468-3"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/17.4.1006"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1515\/sab-2015-0002"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD06134"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/281483a0"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Faji.12720","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.12720","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T11:44:49Z","timestamp":1695987889000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.12720"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,13]]},"references-count":49,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["10.1111\/aji.12720"],"URL":"https:\/\/doi.org\/10.1111\/aji.12720","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,13]]},"article-number":"e12720"}}