{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:04:56Z","timestamp":1791227096717,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,7,25]],"date-time":"2017-07-25T00:00:00Z","timestamp":1500940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000861","name":"Burroughs Wellcome Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000861","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>Placental pericytes are essential for placental microvascular function, stability, and integrity. Mechanisms of human cytomegalovirus (<jats:styled-content style=\"fixed-case\">HCMV<\/jats:styled-content>) pathogenesis incorporating placental pericytes are unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of Study<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">HCMV<\/jats:styled-content>\u2010infected placental tissue was stained by dual\u2010labeled immunohistochemistry. Primary placental pericytes, cytotrophoblasts, and villous fibroblasts were exposed to <jats:styled-content style=\"fixed-case\">HCMV<\/jats:styled-content>; and infectivity was analyzed by microscopy and immunofluorescence. Cytokine expression was examined by Luminex assay. A <jats:styled-content style=\"fixed-case\">HCMV<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content> recombinant virus was used to examine replication kinetics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunohistochemistry showed <jats:styled-content style=\"fixed-case\">HCMV<\/jats:styled-content> in trophoblast and the villous core with T\u2010cell and macrophage infiltration. Primary <jats:styled-content style=\"fixed-case\">HCMV<\/jats:styled-content> isolate from a patient (<jats:styled-content style=\"fixed-case\">SBCMV<\/jats:styled-content>)\u2010 infected pericytes showed dysregulation of proinflammatory and angiogenic cytokines when compared to control cells. A tri\u2010cell model of the villous floor showed a unique expression profile. Finally, we show pericytes infected in vivo with <jats:styled-content style=\"fixed-case\">HCMV<\/jats:styled-content> in placental tissue from a congenitally infected child.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Placental pericytes support <jats:styled-content style=\"fixed-case\">HCMV<\/jats:styled-content> replication, inducing proinflammatory and angiogenic cytokines that likely contribute to viral dissemination, placenta inflammation, and dysregulation of placental angiogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12728","type":"journal-article","created":{"date-parts":[[2017,7,25]],"date-time":"2017-07-25T11:03:48Z","timestamp":1500980628000},"source":"Crossref","is-referenced-by-count":28,"title":["Placental pericytes and cytomegalovirus infectivity: Implications for <scp>HCMV<\/scp> placental pathology and congenital disease"],"prefix":"10.1111","volume":"78","author":[{"given":"David M.","family":"Aronoff","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine Vanderbilt University Medical Center  Nashville TN USA"},{"name":"Department of Pathology, Microbiology and Immunology Vanderbilt University Medical Center  Nashville TN USA"},{"name":"Department of Microbiology and Immunology, Center for AIDS Health Disparities Research Meharry Medical College, School of Medicine  Nashville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hernan","family":"Correa","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine Vanderbilt University Medical Center  Nashville TN USA"},{"name":"Department of Pathology, Microbiology and Immunology Vanderbilt University Medical Center  Nashville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa M.","family":"Rogers","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine Vanderbilt University Medical Center  Nashville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravit","family":"Arav\u2010Boger","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4568-8158","authenticated-orcid":false,"given":"Donald J.","family":"Alcendor","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Center for AIDS Health Disparities Research Meharry Medical College, School of Medicine  Nashville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/GCO.0b013e328342f1f6"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1986.03380140074025"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199202000-00007"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2005.01.059"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jpids\/piu069"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2005.09.015"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2011.07.005"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciq085"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00431-006-0172-6"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu417"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11908-014-0419-8"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-7-71"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu019"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000322394"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161208784705469"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.110.217117"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(86)90026-1"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-014-0219-y"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-9-95"},{"key":"e_1_2_7_21_1","volume-title":"Vascular Biology of the Placenta. Integrated Systems Physiology: From Molecules to Function to Disease: Cell Types of the Placenta","author":"Wang Y","year":"2010"},{"key":"e_1_2_7_22_1","first-page":"43","article-title":"Identifying dysregulated genes induced by Kaposi's sarcoma\u2010associated herpesvirus (KSHV)","volume":"14","author":"Alcendor DJ","year":"2010","journal-title":"J Vis Exp"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1159\/000150506"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix171"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002535"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01415-14"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2006.09.008"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis186"},{"key":"e_1_2_7_29_1","doi-asserted-by":"crossref","first-page":"7598","DOI":"10.1128\/JVI.72.9.7598-7602.1998","article-title":"Human trophoblast cells are permissive to the complete replicative cycle of human cytomegalovirus","volume":"9","author":"Halwachs\u2010Baumann G","year":"1998","journal-title":"J Virol"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2005.02.009"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.24.13301-13314.2003"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1203\/pdr.0b013e318045be6d"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1077"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.15.6808-6820.2000"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1086\/431999"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.6.4970-4979.1998"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2005.02.009"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.13.6710-6717.2002"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0052899"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000147365.86159.f5"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.1.472-485.2005"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/scrt379"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Faji.12728","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.12728","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/aji.12728","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.12728","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T20:50:13Z","timestamp":1694292613000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.12728"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,25]]},"references-count":41,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1111\/aji.12728"],"URL":"https:\/\/doi.org\/10.1111\/aji.12728","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,25]]},"article-number":"e12728"}}