{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T01:45:43Z","timestamp":1780451143942,"version":"3.54.1"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,9,16]],"date-time":"2017-09-16T00:00:00Z","timestamp":1505520000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Institute for Research in Reproductive Health","award":["NIRRH\/RA\/453\/01-2017"],"award-info":[{"award-number":["NIRRH\/RA\/453\/01-2017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>In endocervical epithelial cells (End1\/E6E7), mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> let\u20107f plays an important role in the control of innate immunity. The underlying molecular mechanism for let\u20107f regulation in these cells remains largely unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods of study<\/jats:title><jats:p>let\u20107f was knocked down in End1\/E6E7 cells using si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>, and differential gene expression was analyzed by microarray. Differentially expressed genes were validated by <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> and Western blot. Expression of let\u20107f was studied by <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> after inhibition of C\/<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content>\u03b2 with betulinic acid (<jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">pCMV<\/jats:styled-content>\u03b2 plasmid and after overexpression of C\/<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content>\u03b2 with <jats:styled-content style=\"fixed-case\">pCMV<\/jats:styled-content>\u03b2+ plasmid. Ch<jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content> assay was performed to confirm binding of C\/<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content>\u03b2 to let\u20107f promoter. Levels of Lin28A\/B were checked by <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> after similar treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>let\u20107f knockdown (KD) affects the expression of many transcription factors (eg, C\/<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content>\u03b2) which are important regulators of immune responses. We observed let\u20107f\u20101 promoter to contain 6 C\/<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content>\u03b2 binding sites. KD of C\/<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content>\u03b2 led to decreased let\u20107f expression while overexpression of C\/<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content>\u03b2 increased its expression. Treatment of End1\/E6E7 cells with <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>\u20103 ligand, poly(I:C) increased binding of C\/<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content>\u03b2 at binding sites 3, 5, and 6. Expression of Lin28A\/B also changed upon inhibition and overexpression of C\/<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content>\u03b2. Its expression is opposite to that of let\u20107f in End1\/E6E7 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>let\u20107f\u20101 is a direct target of transcription factor, C\/<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content>\u03b2 in End1\/E6E7 cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12759","type":"journal-article","created":{"date-parts":[[2017,9,18]],"date-time":"2017-09-18T14:11:29Z","timestamp":1505743889000},"source":"Crossref","is-referenced-by-count":4,"title":["Transcription factor <scp>CCAAT<\/scp>\/enhancer\u2010binding protein\u2010\u03b2 upregulates micro<scp>RNA<\/scp>,<i> let\u20107f<\/i>\u20101 in human endocervical cells"],"prefix":"10.1111","volume":"78","author":[{"given":"Kanchana","family":"Ayyar","sequence":"first","affiliation":[{"name":"Division of Molecular Immunology and Microbiology (MIM) National Institute for Research in Reproductive Health (NIRRH)  Mumbai 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