{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T06:46:58Z","timestamp":1771656418353,"version":"3.50.1"},"reference-count":7,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,9,26]],"date-time":"2017-09-26T00:00:00Z","timestamp":1506384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI093692"],"award-info":[{"award-number":["R01AI093692"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["UL1TR00165"],"award-info":[{"award-number":["UL1TR00165"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>Differences in circulating (peripheral) and mucosal T\u2010cell phenotypes in chlamydia\u2010infected women remain largely unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Thirteen paired mononuclear cell specimens from blood and cervicovaginal lavages collected from chlamydia\u2010infected women were stained and analyzed using ten\u2010color cell surface flow cytometry for T\u2010cell distribution, activation status, homing, and T helper (Th)\u2010associated chemokine receptors (<jats:styled-content style=\"fixed-case\">CKR<\/jats:styled-content>s).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A higher proportion of genital mucosal T\u2010cells were activated (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>38<jats:sup>+<\/jats:sup><jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DR<\/jats:styled-content><jats:sup>+<\/jats:sup>) and expressed <jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>5 and Th1\u2010associated <jats:styled-content style=\"fixed-case\">CKR CXCR<\/jats:styled-content>3<jats:sup>+<\/jats:sup><jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>5<jats:sup>+<\/jats:sup> compared to peripheral T\u2010cells, but a lower proportion of mucosal T\u2010cells expressed homing <jats:styled-content style=\"fixed-case\">CKR CCR<\/jats:styled-content>7, Th\u20102 associated <jats:styled-content style=\"fixed-case\">CKR CCR<\/jats:styled-content>4, and <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>3<jats:sup>+<\/jats:sup><jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>4<jats:sup>+<\/jats:sup> for both T\u2010cell subsets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>T\u2010cell phenotypes differed in the peripheral vs genital mucosa compartments in chlamydia\u2010infected women. As chlamydia infects mucosal epithelial cells, the finding of a higher frequency of activated T\u2010cells and Th\u20101 phenotypes in the mucosa likely reflects an adaptive immune response to infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12768","type":"journal-article","created":{"date-parts":[[2017,9,26]],"date-time":"2017-09-26T10:17:10Z","timestamp":1506421030000},"source":"Crossref","is-referenced-by-count":5,"title":["Distinct peripheral vs mucosal T\u2010cell phenotypes in chlamydia\u2010infected women"],"prefix":"10.1111","volume":"78","author":[{"given":"Brian M. O.","family":"Ogendi","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine University of Alabama at Birmingham  Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rakesh K.","family":"Bakshi","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine University of Alabama at Birmingham  Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steffanie","family":"Sabbaj","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine University of Alabama at Birmingham  Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LaDraka'","family":"Brown","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine University of Alabama at Birmingham  Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeannette Y.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biostatistics University of Arkansas for Medical Sciences  Little Rock AR USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richa","family":"Kapil","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine University of Alabama at Birmingham  Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0603-2404","authenticated-orcid":false,"given":"William M.","family":"Geisler","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine University of Alabama at Birmingham  Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,9,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2010.58"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2008.00639.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00539-15"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1502628"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058565"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901216"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.30521"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Faji.12768","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.12768","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T06:20:19Z","timestamp":1695709219000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.12768"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,26]]},"references-count":7,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1111\/aji.12768"],"URL":"https:\/\/doi.org\/10.1111\/aji.12768","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,26]]},"article-number":"e12768"}}