{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:18:06Z","timestamp":1790140686378,"version":"4.0.1"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,12,4]],"date-time":"2017-12-04T00:00:00Z","timestamp":1512345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea.","award":["HI14C0368"],"award-info":[{"award-number":["HI14C0368"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2018,2]]},"abstract":"\n Problem<\/jats:title>\n \n Vitamin D deficiency increases the risk of developing pregnancy\u2010related complications, including preeclampsia and small\u2010for\u2010gestational\u2010age infants. Vitamin D was demonstrated to promote the invasiveness of human extravillous trophoblasts (\n EVT<\/jats:styled-content>\n s). However, whether vitamin D induces the epithelial\u2010mesenchymal transition (\n EMT<\/jats:styled-content>\n ) of\n EVT<\/jats:styled-content>\n s remains unclear. Therefore, we investigated whether vitamin D promotes\n EMT<\/jats:styled-content>\n and the related signaling pathways.\n <\/jats:p>\n <\/jats:sec>\n \n Method of study<\/jats:title>\n \n In this study, we performed\n EMT<\/jats:styled-content>\n experiments using\n JAR<\/jats:styled-content>\n cells based on the expression of the mesenchymal markers and vitamin D receptor.\n JAR<\/jats:styled-content>\n cells were treated with calcitriol, the active form of vitamin D. Western blotting was performed to evaluate\n EMT<\/jats:styled-content>\n markers and key molecules of signaling pathways. Invasion assays were conducted. Expression and secretion of\n MMP<\/jats:styled-content>\n s were analyzed by real\u2010time\n PCR<\/jats:styled-content>\n and zymography.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Calcitriol significantly enhanced\n EMT<\/jats:styled-content>\n and the invasive capability of\n JAR<\/jats:styled-content>\n cells, along with increased expression and secretion of\n MMP<\/jats:styled-content>\n \u20102 and\n MMP<\/jats:styled-content>\n \u20109. Moreover,\n ERK<\/jats:styled-content>\n signaling pathway was activated by calcitriol. The effects of calcitriol were neutralized by\n ERK<\/jats:styled-content>\n signaling blocker.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Calcitriol facilitated\n EMT<\/jats:styled-content>\n induction and expression of\n MMP<\/jats:styled-content>\n s via\n ERK<\/jats:styled-content>\n signaling pathway, which promoted the invasive capability of\n EVT<\/jats:styled-content>\n s. Further studies are warranted to elucidate the potential application of vitamin D in the prevention of pregnancy complications.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/aji.12796","type":"journal-article","created":{"date-parts":[[2017,12,5]],"date-time":"2017-12-05T05:30:41Z","timestamp":1512451841000},"source":"Crossref","is-referenced-by-count":17,"title":["Vitamin D facilitates trophoblast invasion through induction of epithelial\u2010mesenchymal transition"],"prefix":"10.1111","volume":"79","author":[{"given":"Ryang Hee","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Myunggok Medical Research Center College of Medicine Konyang University Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byung Jun","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Myunggok Medical Research Center College of Medicine Konyang University Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ki Mo","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Myunggok Medical Research Center College of Medicine Konyang University Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Won","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Myunggok Medical Research Center College of Medicine Konyang University Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6941-192X","authenticated-orcid":false,"given":"Sung Ki","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Myunggok Medical Research Center College of Medicine Konyang University Daejeon 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