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Eukaryotic initiation factor 5A (<jats:styled-content style=\"fixed-case\">eIF<\/jats:styled-content>5A) is an initiator of translation involved in cellular processes, such as migration, proliferation, and apoptosis. However, the function of <jats:styled-content style=\"fixed-case\">eIF<\/jats:styled-content>5A in trophoblast cells is unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>We inhibited <jats:styled-content style=\"fixed-case\">eIF<\/jats:styled-content>5A and Ca<jats:sup>2+<\/jats:sup>\/calmodulin\u2010dependent protein kinase 1D (<jats:styled-content style=\"fixed-case\">CAMK<\/jats:styled-content>1D) expression in <jats:styled-content style=\"fixed-case\">HTR<\/jats:styled-content>8 cells using <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> interference. The effects of <jats:styled-content style=\"fixed-case\">eIF<\/jats:styled-content>5A and <jats:styled-content style=\"fixed-case\">CAMK<\/jats:styled-content>1D on <jats:styled-content style=\"fixed-case\">HTR<\/jats:styled-content>8 cells were investigated using real\u2010time polymerase chain reaction, Western blotting, flow cytometry, cell transfection assays, cell migration assays, and terminal deoxynucleotidyl transferase <jats:styled-content style=\"fixed-case\">dUTP<\/jats:styled-content> nick\u2010end labeling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">eIF<\/jats:styled-content>5A inhibition decreased <jats:styled-content style=\"fixed-case\">CAMK<\/jats:styled-content>1D expression, proliferation, migration, and invasion, but upregulated apoptosis, in <jats:styled-content style=\"fixed-case\">HTR<\/jats:styled-content>8 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Cross\u2010talk between <jats:styled-content style=\"fixed-case\">eIF<\/jats:styled-content>5A and <jats:styled-content style=\"fixed-case\">CAMK<\/jats:styled-content>1D enhances proliferation, migration, and invasion, but inhibits apoptosis, in trophoblasts.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12845","type":"journal-article","created":{"date-parts":[[2018,3,15]],"date-time":"2018-03-15T11:01:58Z","timestamp":1521111718000},"source":"Crossref","is-referenced-by-count":11,"title":["Eukaryotic initiation factor 5A and Ca<sup>2+<\/sup>\/calmodulin\u2010dependent protein kinase 1D modulate trophoblast cell function"],"prefix":"10.1111","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1464-8428","authenticated-orcid":false,"given":"Xiaoli","family":"Qin","sequence":"first","affiliation":[{"name":"International Peace Maternity and Child Health Hospital, School of Medicine Shanghai Jiao 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