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Adrenally produced glucocorticoids are the body's primary mediators of the inflammatory and stress response. Although the glucocorticoid receptor (GR) is expressed in all human villous trophoblast tissue, the effect of glucocorticoids on placentation is not well understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Using microarray analysis, we identified the glucocorticoid\u2010regulated transcriptional profile in the immortalized first\u2010trimester extravillous trophoblast cell line Swan.71 (Sw.71).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The synthetic glucocorticoid dexamethasone significantly regulated 3829 genes, including genes associated with cell movement, growth, and survival.<jats:italic>SERPINE1<\/jats:italic>, an inhibitor of trophoblast invasion, was induced by glucocorticoids in Sw.71 cells and is associated with the pathogenesis of preeclampsia. Glucocorticoid treatment induced recruitment of activated polymerase II and GR to the<jats:italic>SERPINE1<\/jats:italic>promoter, suggesting a mechanism for transcriptional regulation. Functionally, glucocorticoid treatment inhibited cell proliferation, migration, and invasion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings suggest that glucocorticoids regulate extravillous trophoblast functions by altering the gene expression profile, which may contribute to the pathogenesis of reproductive disorders such as preeclampsia and IUGR.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.12974","type":"journal-article","created":{"date-parts":[[2018,5,18]],"date-time":"2018-05-18T12:11:45Z","timestamp":1526645505000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Glucocorticoid signaling regulates cell invasion and migration in the human first\u2010trimester trophoblast cell line Sw.71"],"prefix":"10.1111","volume":"80","author":[{"given":"Edwina P.","family":"Kisanga","sequence":"first","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive 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