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LncRNA<\/jats:styled-content>s have been identified that play key roles in a variety of human diseases. Recently, lncRNA<\/jats:styled-content>PVT<\/jats:styled-content>1<\/jats:italic> was reported to relate to the pathogenesis and progression of pregnancy. However, the role and underlying mechanism of PVT<\/jats:styled-content>1<\/jats:italic> in trophoblast cell dysfunction in spontaneous abortion remain largely unknown.<\/jats:p><\/jats:sec>Method of study<\/jats:title>The effects of PVT<\/jats:styled-content>1<\/jats:italic>, miR\u2010424, and eIF<\/jats:styled-content>5A on HTR<\/jats:styled-content>8 cells and human villi tissues from spontaneous or induced abortions were investigated using CCK<\/jats:styled-content>\u20108 assay, EdU assay, real\u2010time polymerase chain reaction, Western blotting, cell transfection assays, cell migration assays, and luciferase reporter gene assays.<\/jats:p><\/jats:sec>Results<\/jats:title>Overexpression of PVT<\/jats:styled-content>1<\/jats:italic> promoted HTR<\/jats:styled-content>8 cell viability, proliferation, and migration. Suppression of PVT<\/jats:styled-content>1<\/jats:italic> promoted miR\u2010424 expression, and miR\u2010424 could modulate the effects of PVT<\/jats:styled-content>1<\/jats:italic> in HTR<\/jats:styled-content>8 cells. MiR\u2010424 exerted its function via regulation of eIF<\/jats:styled-content>5A expression in HTR<\/jats:styled-content>8 cells. PVT<\/jats:styled-content>1<\/jats:italic> and eIF<\/jats:styled-content>5A expression were decreased and miR\u2010424 was increased in clinical samples from spontaneous abortion, compared to samples from elective induced abortion.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>PVT<\/jats:styled-content>1<\/jats:italic> regulates trophoblast cell function via modulation of a PVT<\/jats:styled-content>1<\/jats:italic>\/miR\u2010424\/eIF<\/jats:styled-content>5A pathway.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13048","type":"journal-article","created":{"date-parts":[[2018,9,17]],"date-time":"2018-09-17T10:16:33Z","timestamp":1537179393000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Plasmacytoma variant translocation 1 (PVT<\/scp>1<\/i>) regulates trophoblast viability, proliferation, and migration and is downregulated in spontaneous abortion"],"prefix":"10.1111","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1464-8428","authenticated-orcid":false,"given":"Xiaoli","family":"Qin","sequence":"first","affiliation":[{"name":"International Peace Maternity and Child Health Hospital School of Medicine Shanghai Jiao Tong University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Chen","sequence":"additional","affiliation":[{"name":"International Peace Maternity and Child Health Hospital School of Medicine Shanghai Jiao Tong University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shufang","family":"Chen","sequence":"additional","affiliation":[{"name":"International Peace Maternity and Child Health Hospital School of Medicine Shanghai Jiao Tong University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaorui","family":"Liu","sequence":"additional","affiliation":[{"name":"International Peace 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