{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T21:37:41Z","timestamp":1782941861473,"version":"3.54.5"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,11,16]],"date-time":"2018-11-16T00:00:00Z","timestamp":1542326400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"publisher","award":["16ZR1427900"],"award-info":[{"award-number":["16ZR1427900"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571509"],"award-info":[{"award-number":["81571509"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>Endometriosis (EM) is a chronic immunoinflammatory disease associated with an abnormal immunotolerant microenvironment. Myeloid\u2010derived suppressor cells (MDSCs) are a heterogeneous population of immature myeloid cells that play a major role in immunosuppression in cancer, inflammation and other diseases. This paper aims to elucidate whether or not MDSCs are involved in regulating this microenvironment in EM and how this regulation occurs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of Study<\/jats:title><jats:p>Immunochemistry (IHC) and qPCR were conducted to measure CD11b and ARG1 expression in the ectopic endometrium samples from EM patients. CCL25 levels in EM PF and the expression of CCR9 on M\u2010MDSCs were measured by ELISA. M\u2010MDSC migration was determined towards rhCCL25, \u03b1\u2010CCR9, \u03b1\u2010CCL25 and EM PF through in vitro chemotaxis assay. CD33<jats:sup>+<\/jats:sup>CD14<jats:sup>+<\/jats:sup>CD11b<jats:sup>+<\/jats:sup>HLA\u2010DR<jats:sup>\u2010<\/jats:sup> M\u2010MDSCs isolated from EM PBMCs were added to CD8<jats:sup>+<\/jats:sup> T cells stimulated with \u03b1\u2010CD3\/\u03b1\u2010CD28 antibody. After 72\u00a0hours of co\u2010culture, proliferation was measured to rate the immunosuppressive function of M\u2010MDSCs. Finally, levels of IL\u201010, GM\u2010CSF and arginase activity in the cultured supernatants were detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>IHC and qPCR results revealed higher CD11b and ARG1 expression in EM endometrium than normal endometrium. MDSCs accumulated in the EM microenvironment, in which M\u2010MDSCs were the predominant type. CD33<jats:sup>+<\/jats:sup>CD14<jats:sup>+<\/jats:sup>CD11b<jats:sup>+<\/jats:sup>HLA\u2010DR<jats:sup>\u2212<\/jats:sup> M\u2010MDSCs expressed high CCR9 levels and were recruited through CCL25. M\u2010MDSCs from EM PBMCs inhibited proliferation and activity in autologous T cells. rhCCL25 promoted IL\u201010 and GM\u2010CSF secretion and arginase enzymatic activity in CD33<jats:sup>+<\/jats:sup>CD14<jats:sup>+<\/jats:sup>CD11b<jats:sup>+<\/jats:sup>HLA\u2010DR<jats:sup>\u2212<\/jats:sup> M\u2010MDSCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CD33<jats:sup>+<\/jats:sup>CD14<jats:sup>+<\/jats:sup>CD11b<jats:sup>+<\/jats:sup>HLA\u2010DR<jats:sup>\u2212<\/jats:sup> M\u2010MDSCs recruited and activated by CCR9\/CCL25 play a crucial role in the pathogenic progression of endometriosis, thus providing a potential target for EM treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13067","type":"journal-article","created":{"date-parts":[[2018,10,30]],"date-time":"2018-10-30T13:28:18Z","timestamp":1540906098000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["CD33<sup>+<\/sup>CD14<sup>+<\/sup>CD11b<sup>+<\/sup>HLA\u2010DR<sup>\u2212<\/sup> monocytic myeloid\u2010derived suppressor cells recruited and activated by CCR9\/CCL25 are crucial for the pathogenic progression of endometriosis"],"prefix":"10.1111","volume":"81","author":[{"given":"Ya","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Obstetrics &amp; Gynecology, Shanghai General Hospital Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Shao","sequence":"additional","affiliation":[{"name":"Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology 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