{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T15:21:26Z","timestamp":1791559286495,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,11,9]],"date-time":"2018-11-09T00:00:00Z","timestamp":1541721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>Recently, we demonstrated both in vitro and in vivo that an immunological crosstalk between Day\u20107 embryo and immune cells exists locally in the uterus in cows. The peripheral immune response to early embryos at Day\u20107 of pregnancy in cows remains largely unknown. Thus, we aimed to investigate the response of peripheral blood immune cells in the presence of multiple Day\u20107 embryos in the uterus in donor cows of embryo transfer program.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Superovulated cows were either inseminated (n\u00a0=\u00a013) at 12\u201018\u00a0hours post\u2010estrus or remained without inseminations (n\u00a0=\u00a06). Blood was collected following insemination (Day\u20101) and immediately after uterine flushing (Day\u20107). Polymorphonuclear neutrophils (PMNs) and peripheral blood mononuclear cells (PBMCs) were isolated from whole blood for analysis of gene expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Interferon\u2010stimulated genes (<jats:italic>ISGs: ISG15 <\/jats:italic>and<jats:italic> OAS1<\/jats:italic>) were increased in both PMNs and PBMCs, with up\u2010regulation of <jats:italic>PTGES<\/jats:italic> and anti\u2010inflammatory cytokines (<jats:italic>TGFB1<\/jats:italic> and <jats:italic>IL10<\/jats:italic>) expressions at Day\u20107 of post\u2010inseminations in cows, when compared to those at Day\u20107 in non\u2010inseminated cows.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The findings indicate that the presence of multiple embryos in the uterus generates an anti\u2010inflammatory immune response in peripheral blood immune cells at Day\u20107 of pregnancy in cows, which might help to accept semi\u2010allogenic embryo in the uterus.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13069","type":"journal-article","created":{"date-parts":[[2018,10,30]],"date-time":"2018-10-30T16:08:47Z","timestamp":1540915727000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Day\u20107 embryos generate an anti\u2010inflammatory immune response in peripheral blood immune cells in superovulated cows"],"prefix":"10.1111","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6904-8801","authenticated-orcid":false,"given":"Anup K.","family":"Talukder","sequence":"first","affiliation":[{"name":"Graduate School of Animal and Food Hygiene Obihiro University of Agriculture and Veterinary Medicine Obihiro Japan"},{"name":"Department of Gynecology, Obstetrics and Reproductive Health, Faculty of Veterinary Medicine &amp; Animal Science Bangabandhu Sheikh Mujibur Rahman Agricultural University Gazipur Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad B.","family":"Rashid","sequence":"additional","affiliation":[{"name":"Graduate School of Animal and Food Hygiene Obihiro University of Agriculture and Veterinary Medicine Obihiro Japan"},{"name":"Department of Physiology and Pharmacology, Faculty of Veterinary &amp; Animal Science Hajee Mohammad Danesh Science and Technology University Dinajpur Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiro","family":"Takedomi","sequence":"additional","affiliation":[{"name":"Taketomi Reproduction Clinic Obihiro Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Moriyasu","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Group Animal Research Center, Hokkaido Research Organization Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiko","family":"Imakawa","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Sciences Tokai University Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akio","family":"Miyamoto","sequence":"additional","affiliation":[{"name":"Graduate School of Animal and Food Hygiene Obihiro University of Agriculture and Veterinary Medicine Obihiro Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,11,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(93)90285-D"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.1987.tb00775.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0900235"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-26224-8"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2017-056"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.04.178"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/330377a0"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1997.tb00253.x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0175954"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2009-2529"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.1.07015"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.11-094K"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-11-6"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.114.121525"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2017-025"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1350103"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-18-0188"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731115000555"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(92)90153-H"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2016.03.017"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-0863"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.079467"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-10-21"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod59.4.784"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.1.312"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.2527\/2003.8161552x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0034-5288(95)90010-1"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI27306."},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1447-0578.2006.00119.x"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1988.tb00238.x"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1079490"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.8.5163"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2016.83"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.3.1483"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(93)90235-D"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(97)01127-4"},{"key":"e_1_2_9_40_1","doi-asserted-by":"crossref","first-page":"4261","DOI":"10.4049\/jimmunol.154.9.4261","article-title":"IL\u201010 prevents naturally occurring fetal loss in the CBA \u00d7 DBA\/2 mating combination, and local defect in IL\u201010 production in this abortion\u2010prone combination is corrected by in vivo injection of IFN\u2010tau","volume":"154","author":"Chaouat G","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0378(98)00035-7"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Faji.13069","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.13069","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/aji.13069","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/chorus\/v1\/articles\/10.1111%2Faji.13069","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.13069","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T11:08:11Z","timestamp":1694430491000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.13069"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,9]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["10.1111\/aji.13069"],"URL":"https:\/\/doi.org\/10.1111\/aji.13069","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,9]]},"assertion":[{"value":"2018-08-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13069"}}