{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T12:41:10Z","timestamp":1773751270598,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,6,18]],"date-time":"2019-06-18T00:00:00Z","timestamp":1560816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003849","name":"IngaBritt och Arne Lundbergs Forskningsstiftelse","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003849","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004047","name":"Karolinska Institutet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004047","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>Circulating B\u2010cell numbers are lower during pregnancy compared with non\u2010pregnant women, but the underlying reasons for this are unknown. Pregnancy\u2010related hormones could influence B\u2010cell lymphopoiesis in the bone marrow, but B cells may also be recruited to the placenta. To investigate the latter, we examined whether the proportions of total B cells and B cells at different maturational stages in placental intervillous blood (IVB) differ compared with peripheral blood (PB).<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>From 23 paired samples of PB and IVB following full\u2010term healthy pregnancies, total B cells and immature\/transitional, mature\/na\u00efve, and memory B cells were identified by flow cytometry. Chemokine levels in blood were analyzed using a Luminex assay. Placental explant\u2010derived supernatant was assayed for B\u2010cell chemotactic activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The proportions of total B cells and mature\/na\u00efve B cells were significantly higher in IVB relative to PB, while the fractions of immature\/transitional cells and memory B cells were higher in PB. Multivariate factor analysis demonstrated that a specific chemokine profile in IVB, including CCL20, positively associated with higher proportions of mature\/na\u00efve B cells in the intervillous space. All B cells expressed CCR6, the corresponding receptor for CCL20, but the intensity of CCR6 expression was significantly higher in mature\/na\u00efve B cells relative to immature\/transitional B cells. Migration assays showed that placental explant\u2010derived supernatants attract B cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results indicate that B cells, and mature\/na\u00efve B cells in particular, are retained in the intervillous blood in response to certain chemokines produced by the placenta during late healthy pregnancy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13154","type":"journal-article","created":{"date-parts":[[2019,6,5]],"date-time":"2019-06-05T09:14:29Z","timestamp":1559726069000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Mature na\u00efve B cells are retained in the placental intervillous blood and positively associate with specific chemokines in full\u2010term healthy pregnancy"],"prefix":"10.1111","volume":"82","author":[{"given":"Martin","family":"Solders","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine 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