{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T22:51:42Z","timestamp":1789080702193,"version":"build-2803163510"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,8,22]],"date-time":"2019-08-22T00:00:00Z","timestamp":1566432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>We investigated the association between idiopathic recurrent pregnancy loss (RPL) and HLA\u2010<jats:italic>DPB1<\/jats:italic>, HLA\u2010<jats:italic>DQB1<\/jats:italic>, and HLA\u2010<jats:italic>DRB1<\/jats:italic> alleles and <jats:italic>DPB1\u2010DQB1\u2010DRB1<\/jats:italic> haplotypes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Case\u2010control retrospective study involved 93 Lebanese women with unexplained RPL, and 113 multiparous Lebanese women with two or more successful pregnancies, and no miscarriages who served as controls. <jats:italic>DPB1<\/jats:italic>, <jats:italic>DQB1<\/jats:italic>, and <jats:italic>DRB1<\/jats:italic> genotyping was performed by PCR\u2010SSP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Expected and observed <jats:italic>DRB1<\/jats:italic>, <jats:italic>DQB1<\/jats:italic>, and <jats:italic>DPB1<\/jats:italic> frequencies were comparable, and HLA genotype frequencies were in Hardy\u2010Weinberg equilibrium. Significantly higher frequencies of DRB1*04:01:01 and DRB1*08:01:01, and decreased <jats:italic>DRB<\/jats:italic>1*07:01:01 frequency were seen in RPL cases than in controls. On the other hand, the distribution of <jats:italic>DQB1<\/jats:italic> alleles was comparable between cases and control groups. Significantly lower frequencies of DPB1*04:01:01 and DPB1*14:01:01 were seen in women with RPL than control subjects. While the frequency DPB1*02:01:01 was markedly higher in RPL cases than in controls, the difference was not significant. <jats:italic>DPB1\u2010DQB1\u2010DRB1<\/jats:italic> haplotype analysis identified haplotype <jats:italic>DPB1<\/jats:italic>*04:01:01\u2010DQB1*03:02:01\u2010DRB1*04:01:01 to be positively associated, while haplotype DPB1*04:01:01\u2010DQB1*02:01:01\u2010DRB1*07:01:01 to be negatively associated with RPL. Of these two haplotypes, only DPB1*04:01:01\u2010DQB1*02:01:01\u2010DRB1*07:01:01 remained significant after correction for multiple tests (<jats:italic>P<\/jats:italic><jats:sub>c<\/jats:sub>\u00a0=\u00a0.0008).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results confirm an association of select <jats:italic>DRB1<\/jats:italic> and <jats:italic>DPB1<\/jats:italic> alleles with RPL in Lebanese women, and the first to identify <jats:italic>DPB1\u2010DQB1\u2010DRB1<\/jats:italic> linked with altered RPL susceptibility, further highlighting the immunological\/inflammatory nature of RPL.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13173","type":"journal-article","created":{"date-parts":[[2019,7,24]],"date-time":"2019-07-24T13:23:44Z","timestamp":1563974624000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Maternal HLA\u2010DR, HLA\u2010DQ, and HLA\u2010DP loci are linked with altered risk of recurrent pregnancy loss in Lebanese women: A case\u2010control study"],"prefix":"10.1111","volume":"82","author":[{"given":"Gulzhanat","family":"Aimagambetova","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences School of Medicine Nazarbayev University Astana Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelhafidh","family":"Hajjej","sequence":"additional","affiliation":[{"name":"Department of Immunogenetics National Blood Transfusion Center Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zainab H.","family":"Malalla","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry Arabian Gulf University Manama Bahrain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramzi R.","family":"Finan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, H\u00f4tel Dieu de France CHU Universit\u00e9 St. Joseph Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sameh","family":"Sarray","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry Arabian Gulf University Manama Bahrain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1633-9757","authenticated-orcid":false,"given":"Wassim Y.","family":"Almawi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences School of Medicine Nazarbayev University Astana Kazakhstan"},{"name":"Faculte' des Sciences de Tunis Universite' de Tunis El Manar Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,8,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1005330"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/17.2.446"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.06.048"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/hropen\/hoy004"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/17.5.1345"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/del150"},{"key":"e_1_2_8_8_1","unstructured":"Royal College of Obstetricians & Gynaecologists.The investigation and treatment of couples with recurrent first\u2010trimester and second trimester miscarriage. 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