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Few studies examine associations between prenatal immune cell gene expression and gestational length or birthweight. No studies examine which cell types drive associations. The purpose of this study is to explore associations between peripheral blood immune cell gene expression and gestational length and birthweight, using transcript origin analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Eighty\u2010nine women were drawn from the Community Child Health Network cohort. Third trimester maternal dried blood spots were used for genome\u2010wide transcriptional (mRNA) profiling. Gestational length and birthweight were obtained from medical charts. Covariates were age, race\/ethnicity, pre\u2010pregnancy body mass index, smoking, gestational age at blood sampling,\u00a0and pregnancy infections. Associations between gene expression profiles and gestational length and birthweight were tested using general linear models. The Transcription Element Listening System (TELiS) bioinformatics analysis quantified upstream transcription factor activity. Transcript origin analysis identified leukocyte subsets mediating observed effects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Shorter gestation was predicted by increased NF\u2010kB (TFBM ratio\u00a0=\u00a0\u22120.582\u00a0\u00b1\u00a00.172, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001) and monocyte activity (diagnosticity score\u00a0=\u00a00.172\u00a0\u00b1\u00a00.054, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001). Longer gestation was associated with increased dendritic cell activity (diagnosticity score\u00a0=\u00a00.194\u00a0\u00b1\u00a00.039, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001). Increased AP\u20101 activity predicted lower birthweight (TFBM ratio\u00a0=\u00a0\u22120.240\u00a0\u00b1\u00a00.111, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.031). Dendritic cells and CD4+ and CD8+ T cells predicted birthweight\u2010related gene expression differences (diagnosticity score <jats:italic>P<\/jats:italic>'s\u00a0&lt;\u00a00.021).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Higher third trimester pro\u2010inflammatory gene expression predicted shorter gestation and lower birthweight. Variations in monocyte and dendritic cell biology contributed to both effects, and T\u2010cell biology contributed to higher birthweight. These analyses clarify the role of myeloid\/lymphoid lineage immune regulation in pregnancy outcomes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13190","type":"journal-article","created":{"date-parts":[[2019,9,17]],"date-time":"2019-09-17T07:54:41Z","timestamp":1568706881000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Pro\u2010inflammatory immune cell gene expression during the third trimester of pregnancy is associated with shorter gestational length and lower birthweight"],"prefix":"10.1111","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1472-5630","authenticated-orcid":false,"given":"Kharah M.","family":"Ross","sequence":"first","affiliation":[{"name":"Owerko Centre Alberta Children's Hospital Research Institute University of Calgary Calgary Alberta"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith 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