{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:10:59Z","timestamp":1792789859801,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,12,24]],"date-time":"2019-12-24T00:00:00Z","timestamp":1577145600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>Interleukin 35 is a relatively newly discovered cytokine that is produced by regulatory B cells (Bregs) and contributes to their suppressive function, which may contribute to fetal tolerance development and pregnancy maintenance. Therefore, the aim of this study was to determine the frequency of Bregs and expression of IL\u201035 and IL\u201010 in these cells in a normal and abortion\u2010prone murine pregnancy model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods of study<\/jats:title><jats:p>The frequency of Bregs and expression level of IL\u201035 and IL\u201010 in these cells were measured in peripheral blood, uterine draining lymph nodes, uterus, and decidua using flow cytometry. The analysis was performed on days 3 and 14 of pregnancy in normal mice (CBA\/JxBALB\/c) and abortion\u2010prone (CBA\/JxDBA\/2J) murine pregnancy model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A decreased percentage of Breg cells expressing IL\u201035 on day 3 of pregnancy in the uterine draining lymph nodes and in peripheral blood in mice from the abortion group compared with the normal pregnancy group was observed. A similar decrease was also observed in the Breg cells population producing IL\u201010 in peripheral blood. In the uterus (3 dpc) and decidua (14 dpc), a lower percentage of CD19<jats:sup>+<\/jats:sup>IL\u201035<jats:sup>+<\/jats:sup> was also noted in the abortion\u2010prone model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We indicated that the early stages of abortion\u2010prone pregnancy (3 dpc) in mice were characterized by diminished frequency of B cells producing IL\u201035 at both local and peripheral levels. These results and the observed lower level of IL\u201035 in women suffering from recurrent spontaneous abortion suggest that IL\u201035 may be involved in the maintenance of pregnancy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13217","type":"journal-article","created":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T14:56:35Z","timestamp":1575989795000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Regulatory B cells with IL\u201035 and IL\u201010 expression in a normal and abortion\u2010prone murine pregnancy model"],"prefix":"10.1111","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5103-138X","authenticated-orcid":false,"given":"Anna","family":"Slawek","sequence":"first","affiliation":[{"name":"Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences Wroclaw 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