{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:53:22Z","timestamp":1787237602058,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,2,5]],"date-time":"2020-02-05T00:00:00Z","timestamp":1580860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102017R1A6A1A03015713"],"award-info":[{"award-number":["NRF\u20102017R1A6A1A03015713"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Problem<\/jats:title>\n                    <jats:p>Maternal obesity induces elevated saturated fatty acid palmitate levels in the blood and causes pregnancy complications such as gestational diabetes, preeclampsia, fetal growth abnormalities, and stillbirth. Sestrin2, a highly conserved stress\u2010inducible protein, is involved in the cellular responses of various stress conditions and homeostatic regulation. However, the effects of Sestrin2 on trophoblast cells have not yet been investigated. Here, we investigated the role of Sestrin2 in palmitate\u2010induced lipotoxicity and its underlying mechanisms in human first\u2010trimester trophoblast cells (Sw.71).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method of study<\/jats:title>\n                    <jats:p>Mouse placental tissues were obtained from low\u2010fat diet\u2010fed mice (n\u00a0=\u00a014) and high\u2010fat diet\u2010fed mice (n\u00a0=\u00a014) at gestation day 17.5. Sw.71 cells were treated with palmitate or bovine serum albumin as vehicle controls. The role of Sestrin2 in palmitate\u2010induced lipotoxicity was examined by immunocytochemistry, immunoblot analysis, quantitative real\u2010time PCR, and invasion assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Expression of placental Sestrin2 was elevated in high\u2010fat diet\u2010fed dams compared to that of low\u2010fat diet\u2010fed dams. Prolonged treatment of Sw.71 cells with palmitate\u2010induced endoplasmic reticulum (ER) stress\u2010dependent expressions of Sestrin2 protein and mRNA, and the treatment also triggered apoptosis. Knockdown of Sestrin2 increased palmitate\u2010mediated ER stress, inflammatory signaling, and apoptosis. Furthermore, Sestrin2 suppressed impaired trophoblast invasion caused by palmitate and attenuated palmitate\u2010induced ER stress and inflammation via AMPK\/mTORC1 pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our study provides the relationship between Sestrin2, AMPK\/mTORC1 pathway, and trophoblast function, suggesting that Sestrin2 may be a novel potential therapeutic target for the prevention of pregnancy complications.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.13222","type":"journal-article","created":{"date-parts":[[2020,1,20]],"date-time":"2020-01-20T09:11:18Z","timestamp":1579511478000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Sestrin2 alleviates palmitate\u2010induced endoplasmic reticulum stress, apoptosis, and defective invasion of human trophoblast cells"],"prefix":"10.1111","volume":"83","author":[{"given":"Solji","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Cell Biology Konyang University College of Medicine Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiha","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Cell Biology Konyang University College of Medicine Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeji","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Konyang University Hospital Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong Min","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Cell Biology Konyang University College of Medicine Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye 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