{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T11:21:36Z","timestamp":1764588096392,"version":"3.41.2"},"reference-count":62,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,7,11]],"date-time":"2020-07-11T00:00:00Z","timestamp":1594425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>Preterm delivery is the leading cause of neonatal mortality and contributes to delayed physical and cognitive development in children. At present, there is no efficient therapy to prevent preterm labor. A large body of evidence suggests that infections might play a significant and potentially preventable cause of premature birth. This work assessed the effects of erythropoietin (EPO) in a murine model of inflammation\u2010associated preterm delivery, which mimics central features of preterm infections in humans.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>BALB\/c mice were injected i.p. with 20\u00a0000\u00a0IU\/kg EPO or normal saline twice on gestational day (GD) 15, with a 3\u00a0hours time interval between injections. An hour after the first EPO or normal saline injection, all mice received two injections of 50\u00a0\u03bcg\/kg LPS, also given 3\u00a0hours apart.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>EPO significantly prevented preterm labor and increased offspring survival in an LPS induced preterm delivery model. EPO prevented LPS\u2010induced leukocyte infiltration into the placenta. Moreover, EPO inhibited the expression of pro\u2010inflammatory cytokines, interleukin\u20101\u03b2 (IL\u20101\u03b2), interleukin\u20106 (IL\u20106), and tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) in maternal serum and amniotic fluid. EPO also prevented LPS\u2010induced increase in placental prostaglandin (PG)E2 and uterine inducible nitric oxide synthase (iNOS) production, while decreasing nuclear factor kappa\u2010B (NF\u2010\u03ba\u03b2) activity in the myometrium. EPO also increased the gene expression of placental programmed cell death ligand 1 (PD\u2010L1) in LPS\u2010treated mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results suggest that EPO could be a potential novel therapeutic strategy to tackle infection\u2010related preterm labor.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13283","type":"journal-article","created":{"date-parts":[[2020,6,7]],"date-time":"2020-06-07T11:43:52Z","timestamp":1591530232000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Erythropoietin prevents LPS\u2010induced preterm birth and increases offspring survival"],"prefix":"10.1111","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3935-5883","authenticated-orcid":false,"given":"Jie","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Rehabilitation Guangdong Women and Children Hospital  Guangzhou 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