{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:32:09Z","timestamp":1777422729577,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,7,29]],"date-time":"2020-07-29T00:00:00Z","timestamp":1595980800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>Artificial insemination, which requires cryopreservation of semen, is not completely optimized in goats because bucks discharge a small volume of ejaculate and seminal plasma (SP) contains specific proteins that are detrimental to spermatozoa at cryopreservation. However, it is not known the effects of sperm washing (removal of SP) before cryopreservation on the post\u2010thawing frozen spermatozoa of Black Bengal bucks. Moreover, it is completely unknown whether SP of goats contains TGF\u2010\u03b2 and CXCL10 that have been proven essential for fertility in other mammals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty\u2010five ejaculates were collected from six mature Black Bengal bucks at one\u2010week intervals and were subjected to microscopic evaluation for semen characteristics at pre\u2010 and post\u2010freezing condition. The concentrations of TGF\u2010\u03b2 and CXCL10 in the SP using ELISA were determined. SP was harvested with centrifugation of fresh semen at 1500\u00a0<jats:italic>g<\/jats:italic> for 15\u00a0minutes twice at room temperature.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Semen characteristics were significantly varied between bucks. Seminal plasma of all ejaculates contained TGF\u2010\u03b2 and CXCL10 while significant variation of concentrations between bucks was observed in case of CXCL10. Cryopreservation of semen reduced total motility and progressive motility, while sperm washing before cryopreservation was beneficial to the total motility and progressive motility of post\u2010thawing spermatozoa.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Black Bengal buck seminal plasma was affluent of TGF\u2010\u03b2 and CXCL10 and washing of spermatozoa before cryopreservation was beneficial to the post\u2010thawing sperm motility. The results of the current investigation will be helpful for future research on the roles of SP in female reproductive tract and pregnancy in Black Bengal goats.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13301","type":"journal-article","created":{"date-parts":[[2020,7,13]],"date-time":"2020-07-13T18:10:17Z","timestamp":1594663817000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Seminal plasma contains TGF\u2010\u03b2 and CXCL10 but sperm washing before cryopreservation is beneficial for post\u2010thawing sperm motility in Black Bengal goats (<i>Capra hircus<\/i>)"],"prefix":"10.1111","volume":"84","author":[{"given":"Ankon","family":"Das","sequence":"first","affiliation":[{"name":"Department of Surgery and Theriogenology Faculty of Veterinary, Animal and Biomedical Sciences (FVABS) Sylhet Agricultural University (SAU) Sylhet Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"AHM Musleh","family":"Uddin","sequence":"additional","affiliation":[{"name":"Department of Surgery and Theriogenology Faculty of Veterinary, Animal and Biomedical Sciences (FVABS) Sylhet Agricultural University (SAU) Sylhet Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0045-9669","authenticated-orcid":false,"given":"Md Bashir","family":"Uddin","sequence":"additional","affiliation":[{"name":"Department of Medicine FVABS SAU Sylhet Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abu Nasar Md. 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