{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T13:02:32Z","timestamp":1759496552544,"version":"3.41.2"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,9,20]],"date-time":"2020-09-20T00:00:00Z","timestamp":1600560000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>Antibody\u2010mediated autoimmune diseases, such as autoimmune thyroid diseases (ATD), systemic lupus erythematosus (SLE), and antiphospholipid syndrome (APS), often are associated with recurrent fetal loss. One of the ATD is Hashimoto's thyroiditis which recently showed association with complications of pregnancy with increased levels of circulating autoantibodies reactive with epitopes on thyroid tissue such as thyroid peroxidase (anti\u2010TPO). In retrospective study of sera analyses in patients with Hashimoto's thyroiditis, all patients had mainly elevated circulating anti\u2010TPO autoantibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>We assessed the potential of human anti\u2010TPO highly positive IgG, derived from patients with Hashimoto's thyroiditis sera associated with complications of pregnancy, to cause directly complications of pregnancy in murine model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Na\u00efve ICR female mice, infused intravenously with 100\u00a0\u03bcg of anti\u2010TPO\u2010positive IgG, showed increased fetal loss and embryo small for date (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001) in comparison with mice passively transferred with commercial IgG or PBS. Moreover, we observed embryos small for date in the mice passively transferred with anti\u2010TPO\u2010positive IgG, exemplified by reduced weight of embryos and placentae (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.001). Histopathological examination revealed delay in fetal development in 50% cases of anti\u2010TPO\u2010positive IgG\u2010treated mice. Importantly, pathological changes in the transition zone, state of glycogen cells, and significant structural changes in the labyrinth part of placenta were observed in all anti\u2010TPO\u2010positive IgG samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The current study shows in the first time, a direct proof of concept, on the association of human TPO\u2010positive IgG from Hashimoto's thyroiditis patients on fetal loss induction in murine model.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13331","type":"journal-article","created":{"date-parts":[[2020,9,20]],"date-time":"2020-09-20T14:50:47Z","timestamp":1600613447000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The pathogenic role of circulating Hashimoto's Thyroiditis\u2010derived TPO\u2010positive IgG on fetal loss in na\u00efve mice"],"prefix":"10.1111","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7801-7123","authenticated-orcid":false,"given":"Elena","family":"Borodina","sequence":"first","affiliation":[{"name":"Laboratory of the Mosaic of Autoimmunity Department of Obstetrics, Gynecology and Reproductive Sciences Saint Petersburg State University Saint Petersburg Russia"},{"name":"Laboratory of the Mosaic of Autoimmunity Saint Petersburg State University Saint Petersburg Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Itai","family":"Katz","sequence":"additional","affiliation":[{"name":"Zabludowicz Center for Autoimmune Diseases; Sheba Medical Center; Affiliated with the Sackler Faculty of Medicine Tel\u2010Aviv University Tel\u2010Aviv Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Antonelli","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine University of Pisa Pisa Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander M.","family":"Gzgzyan","sequence":"additional","affiliation":[{"name":"Laboratory of the Mosaic of Autoimmunity Department of Obstetrics, Gynecology and Reproductive Sciences Saint Petersburg State University Saint Petersburg Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liailia Kh","family":"Dzhemlikhanova","sequence":"additional","affiliation":[{"name":"Laboratory of the Mosaic of Autoimmunity Department of Obstetrics, Gynecology and Reproductive Sciences Saint Petersburg State University Saint Petersburg Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuri","family":"Ostrinski","sequence":"additional","affiliation":[{"name":"Laboratory of the Mosaic of Autoimmunity Saint Petersburg State University Saint Petersburg Russia"},{"name":"Zabludowicz Center for Autoimmune Diseases; 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